Yazar "Saglam, Necdet" seçeneğine göre listele
Listeleniyor 1 - 7 / 7
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antioxidative and antiproliferative effects of propolis-reduced silver nanoparticles(Techno-Press, 2021) Tan, Gamze; Ilk, Sedef; Foto, Fatma Z.; Foto, Egemen; Saglam, NecdetIn this study, phytochemicals present in Propolis Extract (PE) were employed as reducing and stabilizing reagents to synthesize silver nanoparticles. Three propolis-reduced silver nanoparticles (P-AgNPs1-3) were synthesized using increasing amounts of PE. P-AgNPs were treated with different cancer cells -lung (A549), cervix (HeLa) and colon (WiDr) for 24, 48 and 72 h to evaluate their anti -proliferative activities. A non-cancerous cell type (L929) was also used to test whether suppressive effects of P-AgNPs on cancer cell proliferation were due to a general cytotoxic effect. The characterization results showed that the bioactive contents in propolis successfully induced particle formation. As the amount of PE increased, the particle size decreased; however, the size distribution range expanded. The antioxidant capacity of the particles increased with increased propolis amounts. P-AgNP1 exhibited almost equal inhibitory effects across all cancer cell types; however, P-AgNP2 was more effective on HeLa cells. P-AgNPs3 showed greater inhibitory effects in almost all cancer cells compared to other NPs and pure propolis. Consequently, the biological effects of P-AgNPs were highly dependent on PE amount, NP concentration, and cell type. These results suggest that AgNPs synthesized utilizing propolis phytochemicals might serve as anti -cancer agents, providing greater efficacy against cancer cells.Öğe Bioremediation Applications with Fungi(Springer International Publishing Ag, 2018) Saglam, Necdet; Yesilada, Ozfer; Saglam, Semran; Apohan, Elif; Sam, Mesut; Ilk, Sedef; Emul, Ezgi[Abstract Not Available]Öğe Chitosan nanoparticles enhances the anti-quorum sensing activity of kaempferol(Elsevier Science Bv, 2017) Ilk, Sedef; Saglam, Necdet; Ozgen, Mustafa; Korkusuz, FezaQuorum sensing (QS) is a cell density dependent expression of species in bacteria mediated by compounds called autoinducers (AI). Several processes responsible for successful establishment of bacterial infection are mediated by QS. Inhibition of QS is therefore being considered as a new target for antimicrobial chemotherapy. Flavonoid compounds are strong antioxidant and antimicrobial agents but their applications are limited due to their poor dissolution and bioavailability. Our objective was to investigate the effect of kaempferol loaded chitosan nanoparticles on modulating QS mediated by AI in model bioassay test systems. For this purpose, kaempferol loaded nanoparticles were synthesized and characterized in terms of hydrodynamic diameter, hydrogen bonding, amorphous transformation and antioxidant activity. QS inhibition in time dependent manner of nanoparticles was measured in violacein pigment producing using the biosensor strain Chromobacterium violaceum CV026 mediated by AI known as acylated homoserine lactone (AHL). Our results indicated that the average kaempferol loaded chitosan/TPP nanoparticle size and zeta potential were 192.27 +/- 13.6 nm and +35 mV, respectively. The loading and encapsulation efficiency of kaempferol into chitosan/TPP nanoparticles presented higher values between 78 and 93%. Kaempferol loaded chitosan/TPP nanoparticle during the 30 storage days significantly inhibited the production of violacein pigment in Chromobacterium violaceum CV026. The observation that kaempferol encapsulated chitosan nanoparticles can inhibit QS related processes opens up an exciting new strategy for antimicrobial chemotherapy as stable QS-based anti-biofilm agents. (C) 2016 Elsevier B.V. All rights reserved.Öğe Immobilization of laccase onto a porous nanocomposite: application for textile dye degradation(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Ilk, Sedef; Demircan, Deniz; Saglam, Semran; Saglam, Necdet; Rzayev, Zakir M. O.Poly(MA-alt-MVE)-g-PLA/ODA-MMT nanocomposite was prepared by self-catalytic interlamellar graft copolymerization of L-lactic acid (LA) onto poly(maleic anhydride-alt-methyl vinyl ether) copolymer in the presence of octadecyl amine-montmorillonite (ODA-MMT) organoclay. FTIR, H-1 (C-13) NMR, XRD, and SEM-TEM were utilized for characterizing the resultant nanocomposite. Lactase from Trametes versicolor was immobilized onto the prepared nanocomposite by adsorption or covalent coupling. Decolorization of Reactive Red 3 from aqueous solution by laccase immobilized on the nanocomposite was studied in different conditions (pH, temperature, dye concentration, and reaction time) to investigate the decolorization activity with repeated use and storage. The results indicated that more than 77% of the activity of laccase immobilized systems was retained at the end of 10 cycles. The final decolorization capacity of the immobilized laccase was significantly higher (65%) than that of free laccase (33%) in the chosen optimized conditions (pH 5, 20 degrees C, 0.05 mg/mL laccase concentration, and 90 min).Öğe Innovation of Strategies and Challenges for Fungal Nanobiotechnology(SPRINGER INTERNATIONAL PUBLISHING AG, 2016) Saglam, Necdet; Yesilada, Ozfer; Cabuk, Ahmet; Sam, Mesut; Saglam, Semran; Ilk, Sedef; Gurel, Ekrem; Prasad, RNanotechnology involves the study and use of materials under the 100 nm scale, exploiting the different physiochemical properties exhibited by these materials at the nanoscale level. Microorganisms are the best model and role of action for the nano/biotechnological applications. This technology has become increasingly important for the biotechnology and the related sectors. Promising applications have been already employed in the areas of drug delivery systems using bioactive nanoencapsulation, biosensors to detect and quantify pathogens, chemical and organic compounds, alteration of food compositions, and high-performance sensors and film to preserve fruits and vegetables. Moreover, the taste of food and food safety can be improved by new nano-materials from the microbiological sources. The huge benefits from this technology have led to increases in the market investments in nanoscience and nanoproducts in several areas.Öğe Investigation the potential use of silver nanoparticles synthesized by propolis extract as N-acyl-homoserine lactone-mediated quorum sensing systems inhibitor(Tubitak Scientific & Technological Research Council Turkey, 2020) Ilk, Sedef; Tan, Gamze; Emul, Ezgi; Saglam, NecdetBackground/aim: Quorum sensing (QS) is a chemical communication process that bacteria use to regulate virulence. Inhibition of QS (antiQS) overcomes the pathogenicity of bacteria. Silver nanoparticles (AgNPs) have been used as antimicrobials against pathogens, but have not been used against QS-mediated bacterial infection. Also, studies have been carried out on the inhibitory effects of propolis based structures on pathogen growth, but no studies have been found on their potential use as QS inhibitor. The present study aims to investigate the synthesis and characterization of silver nanoparticles (AgNPs) reduced with propolis extract (P-AgNPs) and evaluation of their antimicrobial and, for the first time, antiQS activity. Materials and methods: P-AgNPs were synthesized using with different volumes (1, 2.5 and 5 mL) of propolis extract (PE) by biological method via reduction of silver nitrate. Synthesized P-AgNPs were characterized in terms of hydrodynamic, chemical, morphological, physical, and antioxidant properties. Disc diffusion and flask incubation assays were used to evaluate the antimicrobial effect against Gram-negative bacteria (Escherichia coli, Proteus mirabilis, Proteus vulgaris, Salmonella typhimurium, Enterobacter aerogenes, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus mutans, Bacillus thuringiensis) and QS-regulated biofilm activity against biosensor strain Chromobacterium violaceum CV026. Results: AgNPs were successfully synthesized by biological method via PE. The violacein pigment production based on the QS system was greatly inhibited by the P-AgNPs (inhibition zones: 16.22-21.48 mm and violacein inhibition: 63.16 +/- 2.4-75.24 +/- 3.5 %) without interfering with the growth of bacteria, which is the first report on the antiQS effect of P-AgNPs. Conclusion: Our results suggest that P-AgNPs may be potentially used to inhibit bacterial physiological processes due to the signal molecules regulates important collective behavior of bacteria. The development of such nontoxic biomaterials may have great potential to evaluate for the new medicinal substance that inhibits the pathogenic biofilms.Öğe Kaempferol loaded lecithin/chitosan nanoparticles: preparation, characterization, and their potential applications as a sustainable antifungal agent(TAYLOR & FRANCIS LTD, 2017) Ilk, Sedef; Saglam, Necdet; Ozgen, MustafaFlavonoid compounds are strong antioxidant and antifungal agents but their applications are limited due to their poor dissolution and bioavailability. The use of nanotechnology in agriculture has received increasing attention, with the development of new formulations containing active compounds. In this study, kaempferol (KAE) was loaded into lecithin/chitosan nanoparticles (LC NPs) to determine antifungal activity compared to pure KAE against the phytopathogenic fungus Fusarium oxysporium to resolve the bioavailability problem. The influence of formulation parameters on the physicochemical properties of KAE loaded lecithin chitosan nanoparticles (KAE-LC NPs) were studied by using the electrostatic self-assembly technique. KAE-LC NPs were characterized in terms of physicochemical properties. KAE has been successfully encapsulated in LC NPs with an efficiency of 93.8 +/- 4.28% and KAE-LC NPs showed good physicochemical stability. Moreover, in vitro evaluation of the KAE-LC NP system was made by the release kinetics, antioxidant and antifungal activity in a time-dependent manner against free KAE. Encapsulated KAE exhibited a significantly inhibition efficacy (67%) against Fusarium oxysporium at the end of the 60 day storage period. The results indicated that KAE-LC NP formulation could solve the problems related to the solubility and loss of KAE during use and storage. The new nanoparticle system enables the use of smaller quantities of fungicide and therefore, offers a more environmentally friendly method of controlling fungal pathogens in agriculture.
