Yeni azometin fenol türevi monomerlerin horseradish peroksidaz enzimi ile oksidatif polimerizasyonu
Yükleniyor...
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Niğde Üniversitesi / Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, ilk olarak azometin fenol türevi 2-[1-(2-fenilhidrazinilidin)etil]fenol ve 2-[(2-fenilhidrazinilidin)metil]fenol monomerleri kondenzasyon reaksiyonu ile sentezlenmiştir ve monomerlerin kimyasal yapısı, Fourier Transform İnfrared Spektroskopisi (FT-IR), Nükleer Manyetik Rezonans Spektroskopisi (1H-NMR, 13C-NMR), Ultraviyole-görünür Bölge Spektroskopisi (UV-vis), Termogravimetrik Analiz (TGA) yöntemleri kullanılarak karakterize edilmiştir. 2-[1-(2-fenilhidrazinilidin)etil] fenol ve 2-[(2-fenilhidrazinilidin)metil]fenol?ün enzimatik oksidatif polimerizasyonu, oksitleyici ajan olarak hidrojen peroksit ve katalizör olarak horseradish peroksidaz (HRP) enzimi kullanılarak oda sıcaklığında farklı çözücü ve farklı fosfat tamponlarında ( pH: 3, 4, 5, 6, 7, 8) yürütülmüştür. Optimum koşullar belirlenmiştir. Elde edilen polimerlerin karakterizasyonu, UV-vis, FT-IR, 1H-NMR, 13C-NMR, TGA, Jel Geçirgenlik Kromatografisi (GPC) ve tarama hızı çalışması ile gerçekleştirilmiştir.
In this study, azomethine phenol derivate 2-[1-(2-phenylhydrazinylidene)ethyl]phenol and 2-[(2-phenylhydrazinylidene)methyl]phenol monomers were firstly synthesized by condensation reaction and the chemical structure of the monomers have been characterized by Fourier Transform İnfrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance Spectroscopy (1H-NMR, 13C-NMR), Ultraviolet-visible Spectroscopy (UV-vis), Thermogravimetric Analysis (TGA) methods. Enzymatic oxidative polymerization of 2-[1-(2-phenylhydrazinylidene)ethyl]phenol and 2-[(2-phenylhydrazinylidene) methyl]phenol using horseradish peroxidase (HRP) enzyme in the presence of hydrogen peroxide as catalyst and oxidizing agent were carried out in various solvents and phosphate buffers (pH: 3, 4, 5, 6, 7, 8) at room temperature. The optimum conditions were determined. Characterization studies of polymers were carried out by UV-Vis, FT-IR, 1H-NMR, 13C-NMR, TGA, Gel Permeation Chromatograpy (GPC) analysis tecniques and study of scan-rate.
In this study, azomethine phenol derivate 2-[1-(2-phenylhydrazinylidene)ethyl]phenol and 2-[(2-phenylhydrazinylidene)methyl]phenol monomers were firstly synthesized by condensation reaction and the chemical structure of the monomers have been characterized by Fourier Transform İnfrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance Spectroscopy (1H-NMR, 13C-NMR), Ultraviolet-visible Spectroscopy (UV-vis), Thermogravimetric Analysis (TGA) methods. Enzymatic oxidative polymerization of 2-[1-(2-phenylhydrazinylidene)ethyl]phenol and 2-[(2-phenylhydrazinylidene) methyl]phenol using horseradish peroxidase (HRP) enzyme in the presence of hydrogen peroxide as catalyst and oxidizing agent were carried out in various solvents and phosphate buffers (pH: 3, 4, 5, 6, 7, 8) at room temperature. The optimum conditions were determined. Characterization studies of polymers were carried out by UV-Vis, FT-IR, 1H-NMR, 13C-NMR, TGA, Gel Permeation Chromatograpy (GPC) analysis tecniques and study of scan-rate.
Açıklama
Anahtar Kelimeler
Azometin Fenol Türevi, Kondenzasyon Reaksiyonu, Enzimatik Oksidatif Polimerizasyon, Horseradish Peroksidaz, Hidrojen Peroksit, Azomethine Phenol Derivate, Condensation Reaction, Enzymatic Oxidative Polymerization, Horseradish Peroksidase, Hydrogen Peroxide
Kaynak
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Sayı
Künye
İşci, İ. (2013). Yeni azometin fenol türevi monomerlerin horseradish peroksidaz enzimi ile oksidatif polimerizasyonu. (Yüksek Lisans Tezi) Niğde Üniversitesi, Fen Bilimleri Enstitüsü, Niğde
