Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment

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dc.authoridHossain, Md Jakir/0000-0003-1076-4867
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridJoyia, Faiz/0000-0002-9540-6972
dc.authoridOZTURK, ZAHIDE NESLIHAN/0000-0002-2592-9997
dc.contributor.authorHossain, Md Jakir
dc.contributor.authorAksoy, Emre
dc.contributor.authorGokce, Neslihan Zahide Ozturk
dc.contributor.authorJoyia, Faiz Ahmad
dc.contributor.authorKhan, Muhammad Sarwar
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:31:58Z
dc.date.available2024-11-07T13:31:58Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractIn contrast with traditional nuclear gene transformation, transplastomic technology has opened a new horizon for the transgenic plant research that offers several beneficial aspects including the convenient use of transgene stacking and the generation of high expression levels of foreign proteins. However, this technology has been well adopted and established in tobacco, the introduction and adoption of cost-effective, swift, and reproducible protocol for in vitro regeneration of transplastomic potato is challenging and laborious. The present research aimed to develop such prompt and efficient protocol to instigate and revive the regeneration potential with the combinations of different plant growth regulators (PGRs). Leaves and internodal explants from four potato cultivars were transformed with chloroplast transformation vector via particle bombardment and cultured on MS media supplemented with suitable PGRs and selection agents. Leaf explants of cultivar Kuroda induced highest (92%) number of calli where cultivar Sante produced the highest (85.7%) transplastomic shoots. Thidiazuron was found more proficient (41%) for shoot regeneration. Finally, within only seven weeks, we got 21 spectinomycin resistant shoot, and 16 of those showed integration of target genes into the plastome in PCR screening.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [216O027]; TUBITAK
dc.description.sponsorshipThis research work is a part of the PhD thesis of Md. Jakir HOSSAIN. The research activities were funded by the Scientific and Technological Research Council of Turkey (TUBITAK, Project No. 216O027). We also acknowledge TUBITAK for financial support to Md. Jakir HOSSAIN to conduct his doctoral research from project funding.
dc.identifier.doi10.3906/tar-2010-103
dc.identifier.endpage323
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85109844300
dc.identifier.scopusqualityQ2
dc.identifier.startpage313
dc.identifier.trdizinid514457
dc.identifier.urihttps://doi.org/10.3906/tar-2010-103
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/514457
dc.identifier.urihttps://hdl.handle.net/11480/15152
dc.identifier.volume45
dc.identifier.wosWOS:000664649600007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectParticle bombardment
dc.subjectgrowth regulators
dc.subjectin vitro regeneration
dc.subjecttransplastomic approach
dc.subjectrapid and cost effective
dc.titleRapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment
dc.typeArticle

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