Poly(acrylic acid)-b-Poly(vinylamine) Copolymer: Decoration with Silver Nanoparticles, Antibacterial Properties, Quorum Sensing Activity, and Cytotoxicity on Breast Cancer and Fibroblast Cell Lines
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Amer Chemical Soc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
An elegant integration of primary amine-bearing segments into the acrylic acid-containing blocks and the subsequent addition of silver nanoparticles (Ag NPs) to this double hydrophilic block copolymer endowed the resulting well-defined self-assembled construct with promising antimicrobial and anti-quorum sensing activity and high cytotoxicity against breast cancer cells. Poly(N- vinylformamide) (PNVF), precursor of amphoteric poly(vinyl amine) (PVAm), was chain-extended from pH-responsive poly(acrylic acid) (PAA) macro-chain transfer agent synthesized via reversible addition-fragmentation chain transfer polymerization by the interchange of xanthates (RAFT/MADIX). PAA-b-PNVF block copolymers with molecular weights in the range of 12500-21800 Da and dispersities between 1.29 and 1.44 were characterized by FTIR, elemental analysis,H- 1 and C-13 nuclear magnetic resonance (NMR) spectroscopy, and size exclusion chromatography (SEC). PAA-b-PVAm copolymer was obtained by hydrolysis of the PNVF block. The decoration of PAA-b-PVAm with monodisperse Ag NPs to yield AgNPs@PAA-b-PVAm proceeded through a simple and green approach by amine-induced reduction of Ag ions in aqueous media. The formation of AgNPs@PAA-b-PVAm was characterized by UV/vis spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and atomic force microscopy (AFM). AgNPs@PAA-b-PVAm displayed superior antimicrobial and anti QS activity. The cell proliferation was tested with an MTT assay on L929 and MCF-7 cell lines. Both the AgNP-decorated and the bare copolymer showed significantly higher cytotoxicity on cancer cells compared to healthy ones.
Açıklama
Anahtar Kelimeler
poly(vinyl amine), poly(acrylic acid), block copolymer, silver nanoparticle, hybrid nanoconstructs, antibacterial, anti-quorum-sensing, cytotoxicity
Kaynak
Acs Applied Polymer Materials
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
4
Sayı
10
