Genomic Variability and Recombination Analysis of Grapevine leafrollassociated virus-1 Isolates from Turkey
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Grapevine leafroll-associated virus-1 (GLRaV-1), one of the causal agents of Grapevine leafroll disease (GLRD), is oneof the most important viral diseases of grapevine worldwide. In this study, the prevalence of GLRaV-1, genetic variationand recombination events among GLRaV-1 isolates in Turkey were investigated. Initially, 197 grapevine samples fromdifferent provinces of the country were serologically tested. Of the total samples, 109 (55.32%) were identified asGLRaV-1 infected. Subsequently, 9 samples representing different geographic distribution were selected for furthersequence analysis of the heat-shock protein 70 homolog (HSP70h), open reading frame 9 (p24), coat protein (CP) andcoat protein duplicate 2 (CPd2). Among the four gene regions, CPd2 was found the most divergent region while HSP70hgene exhibited the lowest genetic diversity. The phylogenetic analysis of four genomic regions including GenBankrecords clustered all variants in two major groups and grouped Turkish isolates mostly together. However, the isolateclusters were not correlated to their geographic origin. Furthermore, several putative recombination events were detectedwith trace to moderate evidence support of algorithms implemented in Recombination Detection Program (RDP). Takentogether, the results provide a better understanding on genetic variation of Turkish GLRaV-1 isolates in the country andworldwide and can help to improve sanitation of propagated material programs for the grape growers.
Açıklama
Anahtar Kelimeler
Biyoloji, Bahçe Bitkileri, Viroloji
Kaynak
Tarım Bilimleri Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
25
Sayı
3
