Vortex-assisted sequential liquid-phase micro-extraction of E127 and E129 in foodstuffs and pharmaceuticals
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the present study, sequential liquid-phase micro-extraction (LPME) method based on amphiphile (1-pentanol) and ion-pair agent as the green chemicals for micro-separation, preconcentration and UV-vis micro-spectrophotometric determination of Erythrosine (E127) and Allura red (E129) in foodstuffs and pharmaceuti-cals was developed for the first time. 1-pentanol was used as extraction solvent for micro-extraction of E127 and to prepare alkanol based ion-pair solvent with tetra-pentyl ammonium bromide for micro-extraction of E129. E127 and E129 were determined by spectrophotometer at 538 and 506 nm, respectively. Micro-separation and micro-extraction parameters of the methods including pH, type and volume of 1-pentanol and ion-pair solvent, time of vortex and centrifugation were examined in detail and optimized. At the optimum conditions, inter-ference effects of matrix components and effect of sample volume on the micro-extraction methods were investigated. Limit of detection and limit of quantification were found as 3.2 mu g/L, 8.4 mu g/L for E127 and 5.2 mu g/ L, 13.7 mu g/L for E129, respectively. 15 preconcentration factor was attained for each of the dyes. The possible extraction mechanisms of the alkanol based micro-extraction for E127 and ion-pair agent based micro-extraction for E129 were elucidated in detail and also showed with images. Finally, the proposed sequential micro -separation technique was validated by analyte addition and recovery studies and applied to determine E127 and E129 contents of commercially available foodstuffs and pharmaceuticals in Turkish markets and pharmacies.
Açıklama
Anahtar Kelimeler
Allura red, Erythrosine, E127, E129, Ion-pair agent, Liquid-phase micro-extraction, 1-pentanol, UV-visible spectrophotometry
Kaynak
Microchemical Journal
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
187
