RESOLVIN D1 (RvD1) REGULATES PORPHYROMONAS GINGIVALIS LIPOPOLYSACCHARIDE-INDUCED Del-1 AND CYTOKINE EXPRESSIONS IN HUMAN GINGIVAL FIBROBLASTS
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dokuz Eylul Univ Inst Health Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: To detect the effect of Resolvin D1 (RvD1) on Developmental endothelial locus-1 (Del-1) and cytokine expressions of human gingival fibroblast cells exposed to Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS).Material and Methods: The effect of RvD1 on cell viability of human gingival fibroblasts exposed to P. gingivalis-LPS was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Meanwhile, the effect of RvD1 on Del-1 and cytokine (IL-1 & beta;, IL-6, IL-8, IL-10, IL-17) expressions of human gingival fibroblasts exposed to P. gingivalis-LPS (1000 ng/mL) were studied by real-time PCR experiment, statistical analysis was performed using GraphPad Prism version 5 for Windows.Results: Cell viability assay results demonstrated that RvD1 concentrations upregulated cell number compared to control group at 24 and at 72 h. While RvD1 reduced the IL-6, IL-8, and IL-17 mRNA expressions, the IL-10 and Del-1 mRNA expressions increased in a time-and dose-dependent manner. Also, IL-1 & beta; was not affected by RvD1 treatments.Conclusion: The increased expression of Del-1 and IL-10 by RvD1 down-regulated the pro-inflammatory cytokine expressions induced by P. gingivalis-LPS in gingival fibroblast. Resolvin D1displayed regulatory effects on gingival inflammation in P. gingivalis LPS-induced cell culture experiment. In particular, results of this study show that Del-1 induced by RvD1 may have therapeutic potential to modulate periodontal inflammation.
Açıklama
Anahtar Kelimeler
Cytokine, Del-1, gingival fibroblast, resolvin D1
Kaynak
Journal of Basic and Clinical Health Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
7
Sayı
2
