RESOLVIN D1 (RvD1) REGULATES PORPHYROMONAS GINGIVALIS LIPOPOLYSACCHARIDE-INDUCED Del-1 AND CYTOKINE EXPRESSIONS IN HUMAN GINGIVAL FIBROBLASTS

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dc.authoridBozkurt, Serife Buket/0000-0002-8641-2844
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorHakki, Sema S.
dc.date.accessioned2024-11-07T13:32:15Z
dc.date.available2024-11-07T13:32:15Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPurpose: To detect the effect of Resolvin D1 (RvD1) on Developmental endothelial locus-1 (Del-1) and cytokine expressions of human gingival fibroblast cells exposed to Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS).Material and Methods: The effect of RvD1 on cell viability of human gingival fibroblasts exposed to P. gingivalis-LPS was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Meanwhile, the effect of RvD1 on Del-1 and cytokine (IL-1 & beta;, IL-6, IL-8, IL-10, IL-17) expressions of human gingival fibroblasts exposed to P. gingivalis-LPS (1000 ng/mL) were studied by real-time PCR experiment, statistical analysis was performed using GraphPad Prism version 5 for Windows.Results: Cell viability assay results demonstrated that RvD1 concentrations upregulated cell number compared to control group at 24 and at 72 h. While RvD1 reduced the IL-6, IL-8, and IL-17 mRNA expressions, the IL-10 and Del-1 mRNA expressions increased in a time-and dose-dependent manner. Also, IL-1 & beta; was not affected by RvD1 treatments.Conclusion: The increased expression of Del-1 and IL-10 by RvD1 down-regulated the pro-inflammatory cytokine expressions induced by P. gingivalis-LPS in gingival fibroblast. Resolvin D1displayed regulatory effects on gingival inflammation in P. gingivalis LPS-induced cell culture experiment. In particular, results of this study show that Del-1 induced by RvD1 may have therapeutic potential to modulate periodontal inflammation.
dc.identifier.doi10.30621/jbachs.1141356
dc.identifier.endpage643
dc.identifier.issn2458-8938
dc.identifier.issn2564-7288
dc.identifier.issue2
dc.identifier.startpage635
dc.identifier.urihttps://doi.org/10.30621/jbachs.1141356
dc.identifier.urihttps://hdl.handle.net/11480/15315
dc.identifier.volume7
dc.identifier.wosWOS:001019553000011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherDokuz Eylul Univ Inst Health Sciences
dc.relation.ispartofJournal of Basic and Clinical Health Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectCytokine
dc.subjectDel-1
dc.subjectgingival fibroblast
dc.subjectresolvin D1
dc.titleRESOLVIN D1 (RvD1) REGULATES PORPHYROMONAS GINGIVALIS LIPOPOLYSACCHARIDE-INDUCED Del-1 AND CYTOKINE EXPRESSIONS IN HUMAN GINGIVAL FIBROBLASTS
dc.typeArticle

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