Effects of ?-lactalbumin and sulindac on primary and metastatic human colon cancer cell lines

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dc.contributor.authorAydemir, Isil
dc.contributor.authorVatansever, Seda
dc.contributor.authorOzbilgin, Kemal
dc.date.accessioned2024-11-07T13:34:06Z
dc.date.available2024-11-07T13:34:06Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: HAMLET (human alpha-lactalbumin made lethal to tumor cells) and sulindac are active ingredients which are used in the treatment of cancers. Under both in vivo and in vitro conditions, HAMLET binds to the surface of tumor cells and enters the cells. Sulindac is one of nonsteroidal anti-inflammatory drugs. It inhibits the growth of tumor cells by inducing the apoptosis. In our study, we aimed to investigate effects of alpha-lactalbumin and sulindac on COLO-320 primary and COLO-741 metastatic human colon carcinoma cell lines via mitochondrial apoptotic pathway by indirect immunocytochemistry and TUNEL assays. Materials and methods: The effects of alpha-lactalbumin and sulindac were assessed by using MTT assay at 24, 48 and 72 h and apoptosis markers caspase-3, caspase-9 and cytochrome-c were detected using immunocytochemistry and TUNEL methods. Results: It was appeared that alpha-lactalbumin and sulindac may triggger mechanisms of apoptosis in both primary and metastatic colon carcinoma cell lines and the primary colon carcinoma cell line was affected more than the metastatic cells. Conclusion: It is proposed that alpha-lactalbumin and sulindac can be used in cancer treatments and future in vivo experiments.
dc.description.sponsorshipScientific Research Committee of Celal Bayar University [SAGBE/2007-052]
dc.description.sponsorshipThis study was supported by the Scientific Research Committee of Celal Bayar University (SAGBE/2007-052). We would like to special thanks to Prof. Dr. Alper Bagriyanik from Dokuz Eylul University, Faculty of Medicine, Department of Histology and Embryology, Izmir, Turkey for examination of electron micrographs.
dc.identifier.doi10.1515/tjb-2017-0263
dc.identifier.endpage796
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85077627370
dc.identifier.scopusqualityQ4
dc.identifier.startpage787
dc.identifier.trdizinid340496
dc.identifier.urihttps://doi.org/10.1515/tjb-2017-0263
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/340496
dc.identifier.urihttps://hdl.handle.net/11480/15795
dc.identifier.volume44
dc.identifier.wosWOS:000501564800009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectApoptosis
dc.subjectCancer
dc.subjectIn vitro
dc.subjectalpha-Lactalbumin
dc.subjectSulindac
dc.titleEffects of ?-lactalbumin and sulindac on primary and metastatic human colon cancer cell lines
dc.typeArticle

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