Estrogen as a novel agent for induction of adipose-derived mesenchymal stem cells for osteogenic differentiation: In vivo bone tissue-engineering study
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Lippincott Williams and Wilkins
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BACKGROUND: This study investigated whether the in vivo osteogenic differentiation potential of adipose-derived mesenchymal stem cells is enhanced by 17?-estradiol. METHODS: Thirty Sprague-Dawley rats were randomized and divided into five experimental groups. For the surgical procedure, biparietal full-thickness bone defects (7 mm in diameter) were created. A chitosan-hydroxyapatite scaffold was used as the vehicle system for 17?-estradiol-loaded nanoparticles and adipose-derived mesenchymal stem cells. The first group, the blank defect group, was the control group. The defects were filled with either scaffold, estradiol, and scaffold; scaffold and adipose-derived mesenchymal stem cells; or estradiol, scaffold, and adipose-derived mesenchymal stem cells as experimental groups. The rats were killed at the end of weeks 4 and 12, and their calvariae were harvested for histologic and microtomographic evaluation. RESULTS: Micro-computed tomographic evaluation of estradiol, scaffold, and adipose-derived mesenchymal stem cells revealed the highest median value (82.59 ± 17.17), and the difference was significant compared with the blank defect group (p = 0.004). Histologic samples demonstrated a significant difference between experimental groups for bone defect repair at the end of weeks 4 and 12 (p = 0.003 and p < 0.001). The estradiol, scaffold, and adipose-derived mesenchymal stem cell group had the highest median score (3.00 ± 0.0) at week 12, which was significantly higher than scores for the scaffold and adipose-derived mesenchymal stem cell group and the blank defect group. CONCLUSION: 17?-Estradiol appears to be a novel and promising agent for future cell-based bone tissue-engineering studies. Copyright © 2014 by the American Society of Plastic Surgeons.
Açıklama
Anahtar Kelimeler
Animals, Cell Differentiation, Estradiol, Mesenchymal Stromal Cells, Nanoparticles, Osteogenesis, Rats, Rats, Sprague-Dawley, Tissue Engineering, Tissue Scaffolds, estradiol, nanoparticle, tissue scaffold, animal, article, bone development, cell differentiation, drug effect, mesenchymal stroma cell, physiology, rat, Sprague Dawley rat, tissue engineering
Kaynak
Plastic and Reconstructive Surgery
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
133
Sayı
4
