Estrogen as a novel agent for induction of adipose-derived mesenchymal stem cells for osteogenic differentiation: In vivo bone tissue-engineering study

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dc.contributor.authorCalis, Mert
dc.contributor.authorDemirtas, Tugrul Tolga
dc.contributor.authorAtilla, Pergin
dc.contributor.authorTatar, Ilkan
dc.contributor.authorErsoy, Orkun
dc.contributor.authorIrmak, Gulseren
dc.contributor.authorCelik, Hakan Hamdi
dc.date.accessioned2024-11-07T10:39:36Z
dc.date.available2024-11-07T10:39:36Z
dc.date.issued2014
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBACKGROUND: This study investigated whether the in vivo osteogenic differentiation potential of adipose-derived mesenchymal stem cells is enhanced by 17?-estradiol. METHODS: Thirty Sprague-Dawley rats were randomized and divided into five experimental groups. For the surgical procedure, biparietal full-thickness bone defects (7 mm in diameter) were created. A chitosan-hydroxyapatite scaffold was used as the vehicle system for 17?-estradiol-loaded nanoparticles and adipose-derived mesenchymal stem cells. The first group, the blank defect group, was the control group. The defects were filled with either scaffold, estradiol, and scaffold; scaffold and adipose-derived mesenchymal stem cells; or estradiol, scaffold, and adipose-derived mesenchymal stem cells as experimental groups. The rats were killed at the end of weeks 4 and 12, and their calvariae were harvested for histologic and microtomographic evaluation. RESULTS: Micro-computed tomographic evaluation of estradiol, scaffold, and adipose-derived mesenchymal stem cells revealed the highest median value (82.59 ± 17.17), and the difference was significant compared with the blank defect group (p = 0.004). Histologic samples demonstrated a significant difference between experimental groups for bone defect repair at the end of weeks 4 and 12 (p = 0.003 and p < 0.001). The estradiol, scaffold, and adipose-derived mesenchymal stem cell group had the highest median score (3.00 ± 0.0) at week 12, which was significantly higher than scores for the scaffold and adipose-derived mesenchymal stem cell group and the blank defect group. CONCLUSION: 17?-Estradiol appears to be a novel and promising agent for future cell-based bone tissue-engineering studies. Copyright © 2014 by the American Society of Plastic Surgeons.
dc.identifier.endpage510e
dc.identifier.issn0032-1052
dc.identifier.issue4
dc.identifier.pmid24675202
dc.identifier.scopus2-s2.0-84898722635
dc.identifier.scopusqualityQ1
dc.identifier.startpage499e
dc.identifier.urihttps://hdl.handle.net/11480/11077
dc.identifier.volume133
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherLippincott Williams and Wilkins
dc.relation.ispartofPlastic and Reconstructive Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectEstradiol
dc.subjectMesenchymal Stromal Cells
dc.subjectNanoparticles
dc.subjectOsteogenesis
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectTissue Engineering
dc.subjectTissue Scaffolds
dc.subjectestradiol
dc.subjectnanoparticle
dc.subjecttissue scaffold
dc.subjectanimal
dc.subjectarticle
dc.subjectbone development
dc.subjectcell differentiation
dc.subjectdrug effect
dc.subjectmesenchymal stroma cell
dc.subjectphysiology
dc.subjectrat
dc.subjectSprague Dawley rat
dc.subjecttissue engineering
dc.titleEstrogen as a novel agent for induction of adipose-derived mesenchymal stem cells for osteogenic differentiation: In vivo bone tissue-engineering study
dc.typeArticle

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