Effects of vitamin B12 on methotrexate hepatotoxicity: evaluation of receptor-interacting protein (RIP) kinase

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dc.contributor.authorKarabulut, Derya
dc.contributor.authorOzturk, Emel
dc.contributor.authorKuloglu, Nurhan
dc.contributor.authorAkin, Ali Tugrul
dc.contributor.authorKaymak, Emin
dc.contributor.authorYakan, Birkan
dc.date.accessioned2024-11-07T13:34:53Z
dc.date.available2024-11-07T13:34:53Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractIn the study, we aimed to show the effects of vitamin B12 on the necrosis caused by methotrexate (MTX), a folic acid antagonist. Thirty-two rats were randomly assigned to four groups of eight rats per group. Control (n= 8), Vit B12 (n= 8) 3 mu g/kg/ip B12 (15 days) per day throughout the experiment, MTX (n= 8) injected with a single dose of 20 mg/kg/ip MTX on 8th day of experiment, MTX + Vit B12 (n= 8) injected with a single dose of 20 mg/kg ip methotrexate on 8th day of experiment + 3 mu g/kg/ip Vit B12 (15 days) per day throughout the experiment. Oxidant (TOS)/antioxidant (TAS) system, TNF-alpha and TGF-beta levels, AST and ALT, serum vitamin B12 levels were determined in the tissue. Cyclooxygenase-2 (Cox-2), receptor-interacting protein kinase 1 (RIP1) and 3 (RIP3) immunohistochemistry were applied to the liver tissue. TOS increased; TAS decreased; TNF-alpha and TGF-beta levels increased; AST and ALT levels changed after MTX hepatotoxicity. Vit B12 decreased significantly. COX-2, RIP1, and RIP3 immunoreactivity increased. Vit B12 showed improvement in all of the negative results. Vit B12 is an important supplement to be used against necrosis in tissue after MTX hepatotoxicity.
dc.description.sponsorshipErciyes University the Scientific Research Projects Unit [TSA-2019-8673]
dc.description.sponsorshipThis work was supported by Erciyes University the Scientific Research Projects Unit, TSA-2019-8673 project code.
dc.identifier.doi10.1007/s00210-020-01992-1
dc.identifier.endpage2480
dc.identifier.issn0028-1298
dc.identifier.issn1432-1912
dc.identifier.issue12
dc.identifier.pmid33052426
dc.identifier.scopus2-s2.0-85092534574
dc.identifier.scopusqualityQ2
dc.identifier.startpage2473
dc.identifier.urihttps://doi.org/10.1007/s00210-020-01992-1
dc.identifier.urihttps://hdl.handle.net/11480/16227
dc.identifier.volume393
dc.identifier.wosWOS:000577243100002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofNaunyn-Schmiedebergs Archives of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectReceptor-interacting protein kinase
dc.subjectMethotrexate
dc.subjectVitamin B12
dc.titleEffects of vitamin B12 on methotrexate hepatotoxicity: evaluation of receptor-interacting protein (RIP) kinase
dc.typeArticle

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