First report of Phytophthora crown and root rot of cherry caused by Phytophthora palmivora in eastern Turkey
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TAYLOR & FRANCIS INC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Crown and root rot or decline is an increasing problem on sweet cherry in Mardin, Malatya, Elaz and Diyarbakr provinces in eastern Turkey. A survey was carried out during 2012-2014 in 120 commercial cherry orchards for disease symptoms, which included poor growth with sparse off-colour foliage, reddish-brown discolouration of the crown and roots, wilting and dieback of the canopy, and in many cases, tree death. Fifty-eight Phytophthora isolates obtained during the course of this 3-year survey were examined and identified as Phytophthora palmivora on the basis of morphological characteristics. BLAST analysis of ITS region sequences of rDNA of five isolates revealed 99-100% identity with reference isolates of P. palmivora from GenBank and Phytophthora database. Isolates of P. palmivora were pathogenic on 12-month-old potted Mahaleb' cherry (Prunus mahaleb L.) rootstock seedlings that were wound-inoculated on the roots and on the crown. Inoculated plants expressed similar symptoms to those observed in the field and almost all infected plants died within 3 months after transplanting. This study demonstrated that P. palmivora is the causal agent of cherry decline in eastern Turkey. To our knowledge, this is the first report of P. palmivora on this host plant. ResumeLa pourriture du collet et des racines, ou deperissement, constitue un probleme croissant chez le cerisier sauvage dans les provinces de Mardin, de Malatya, d'Elaz et de Diyarbakr de l'est de la Turquie. Une etude a ete menee de 2012 a 2014 dans 120 vergers commerciaux afin de deceler les symptomes de la maladie qui incluent la croissance mediocre accompagnee d'un feuillage clairseme de couleur atypique, la coloration brun-rougeatre du collet et des racines, le fletrissement et le deperissement de la cime et, dans plusieurs cas, la mort de l'arbre. Durant les trois annees qu'a dure cette etude, 58 isolats de Phytophthora ont ete examines et identifies, sur la base de leurs caracteres morphologiques, en tant que Phytophthora palmivora. L'analyse de type BLAST des sequences de l'espaceur transcrit interne de l'ADNr de cinq isolats a revele un taux d'identite de 99% a 100% par rapport aux isolats de reference de P. palmivora provenant de la GenBank et de la Phytophthora database. Les isolats de P. palmivora etaient virulents a l'egard de jeunes plants en pots de 12 mois sur porte-greffe de cerisier Mahaleb' (Prunus mahaleb L.) dont des blessures aux collets et aux racines avaient ete inoculees. Les plants inocules ont affiche des symptomes semblables a ceux observes sur le terrain, et presque tous les plants sont morts en moins de trois mois apres avoir ete transplantes. Cette etude a demontre que P. palmivora est l'agent causal du deperissement des cerisiers dans l'est de la Turquie. Pour autant que nous le sachions, il s'agit de la premiere mention de P. palmivora s'attaquant a cette plante hote.
Açıklama
Anahtar Kelimeler
dieback, Phytophthora palmivora, Prunus avium, rot, sweet cherry
Kaynak
CANADIAN JOURNAL OF PLANT PATHOLOGY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
37
Sayı
3
