A comprehensive study on the molecular characterization of tomato spotted wilt orthotospovirus isolates and resistance genes in pepper and tomato

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dc.contributor.authorSajid, Qurat Ul Ain
dc.contributor.authorElci, Eminur
dc.date.accessioned2024-11-07T13:24:14Z
dc.date.available2024-11-07T13:24:14Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractTomato spotted wilt orthotospovirus (TSWV, Orthotospovirus tomatomaculae) resistance genes (Tsw, Sw5) have been identified in pepper and tomato plants. The development of resistant cultivars is one of the popular management strategies for overcoming viral infections. However, the breakdown of resistance genes has been documented from many places where resistant cultivars have been developed. This has mainly been due to the emergence of resistance-breaking (RB) lines because of the consecutive use of these cultivars. The development of these isolates may cause great losses in both tomato and pepper plants in Turkiye. To investigate the incidence of TSWV, 150 symptomatic samples were collected from widespread locations of tomato and pepper growing provinces in Turkiye. Samples were inoculated to enhance the virus titer and further confirmed by enzyme-linked immunosorbent assay (ELISA). The ELISA positive samples were confirmed by reverse transcription-polymerase chain reaction. The sequence-characterized amplified region (SCAR) and CAPS markers were used to screen resistance genes in tomato and pepper plants. Positive TSWV-isolates were partially sequenced to determine whether they had the previously reported mutations in NSs or NSm genes for RB isolates. As per the virus characterization, NSm and NSs amino acid sequences showed no previously identified mutations. Resistance gene-carrying isolates were amplified for partial genome sequences. Phylogenetic analyses revealed that both NSs and NSm were distributed in the same genetic pool. The N gene sequence comparison and phylogenetic analysis showed that the Turkish isolates were clustered in a separate clade. These findings provide insight into TSWV infections and resistance genes of pepper and tomato by phylogenetic analysis.
dc.description.sponsorshipScientific Research Projects Unit (BAP project) [TGT 2020/7 -BAGEP]
dc.description.sponsorshipThe current research was part of the Doctoral Thesis of Qurat ul Ain SAJID, which was partially supported by the Scientific Research Projects Unit (BAP project no. TGT 2020/7 -BAGEP) of Nigde amer Halisdemir University from Nigde, Tuerkiye.
dc.identifier.doi10.55730/1300-008X.2791
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85183903719
dc.identifier.scopusqualityQ2
dc.identifier.trdizinid1239598
dc.identifier.urihttps://doi.org/10.55730/1300-008X.2791
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1239598
dc.identifier.urihttps://hdl.handle.net/11480/13993
dc.identifier.volume48
dc.identifier.wosWOS:001166161300002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectTomato
dc.subjectpepper
dc.subjectresistance
dc.subjectmolecular marker
dc.subjectmovement protein
dc.subjectsilencing suppressor protein
dc.titleA comprehensive study on the molecular characterization of tomato spotted wilt orthotospovirus isolates and resistance genes in pepper and tomato
dc.typeArticle

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