Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p

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dc.authoridDemirel, Ufuk/0000-0002-3457-5086
dc.authoridCALISKAN, MEHMET EMIN/0000-0002-4703-8853
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridtindas, ilknur/0000-0003-3036-7121
dc.authoridOZTURK, ZAHIDE NESLIHAN/0000-0002-2592-9997
dc.authoridAsim, Arslan/0000-0003-1840-0136
dc.contributor.authorAsim, Arslan
dc.contributor.authorGokce, Zahide Neslihan Ozturk
dc.contributor.authorBakhsh, Allah
dc.contributor.authorCayli, Ilknur Tindas
dc.contributor.authorAksoy, Emre
dc.contributor.authorCsliskan, Sevgi
dc.contributor.authorCaliskan, Mehmet Emin
dc.date.accessioned2024-11-07T13:34:49Z
dc.date.available2024-11-07T13:34:49Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractMicroRNAs (miRNAs) are essential players of plant defence systems because of their involvement in reprogramming gene expression under adverse environmental conditions including drought and heat, which are considered major players in limiting crop productivity. miR172b-3p was previously determined as a remarkable stress-responsive miRNA in our next-generation sequencing (NGS) analysis in potato. This study aims to understand the functions of miR172b-3p and its target (ERTF RAP2-7-like) under drought, heat, and combined treatments by overexpressing the miR172b-3p in stress-tolerant (Unica) and sensitive (Russet Burbank) potato cultivars. miR172b-3p overexpression in transgenic lines suppressed the ERTF RAP2-7-like expression leading to enhanced carbon fixation efficiency. Meanwhile, the accumulation of hydrogen peroxide (H2O2) was reduced in both cultivars, proving that it is involved in the front-line tolerance mechanism against individual drought, heat, and their combination. In conclusion, our results prove that the stress tolerance could be enhanced by miR172b-3p-mediated negative regulation of ERTF RAP2-7-like gene in potato under drought, heat, and their combination. Our findings represent the first step towards the improvement of tolerance against multiple abiotic stresses in potato.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [115O405]
dc.description.sponsorshipThe present research work is part of Mr. Arslan Asim's doctoral thesis and has been supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under grant no. 115O405. The authors thank Begimay Taalaybek Kizi, Beyazit Abdurrahman Sanli, Caner Yavuz, Esra Kaplan, Melis Yalcin, Muhammad Naeem, and Mehtap Vural for their help in this study.
dc.identifier.doi10.3906/tar-2103-60
dc.identifier.endpage+
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85119838593
dc.identifier.scopusqualityQ2
dc.identifier.startpage651
dc.identifier.trdizinid514602
dc.identifier.urihttps://doi.org/10.3906/tar-2103-60
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/514602
dc.identifier.urihttps://hdl.handle.net/11480/16174
dc.identifier.volume45
dc.identifier.wosWOS:000708964700010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectAbiotic stress
dc.subjectERTF RAP2-7-like
dc.subjectmiR172b-3p
dc.subjectoverexpression
dc.subjectSolanum tuberosum
dc.titleIndividual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p
dc.typeArticle

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