Colonization of Beauveria bassiana 08F04 in root-zone soil and its biocontrol of cereal cyst nematode (Heterodera filipjevi)

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dc.authoridRiley, Ian Timothy/0000-0002-3592-0785
dc.contributor.authorZhang, Jie
dc.contributor.authorFu, Bo
dc.contributor.authorLin, Qitong
dc.contributor.authorRiley, Ian T.
dc.contributor.authorDing, Shengli
dc.contributor.authorChen, Linlin
dc.contributor.authorCui, Jiangkuan
dc.date.accessioned2024-11-07T13:32:48Z
dc.date.available2024-11-07T13:32:48Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractCereal cyst nematodes cause serious yield losses of wheat in Hunaghuai winter wheat growing region in China. Beauveria bassiana 08F04 isolated from the surface of cysts is a promising biological control agent for cereal cyst nematodes. As the colonization capacity is a crucial criteria to assess biocontrol effectiveness for a microbial agent candidate, we aimed to label B. bassiana 08F04 for efficient monitoring of colonization in the soil. The binary pCAM-gfp plasmid containing sgfp and hph was integrated into B. bassiana 08F04 using the Agrobacterium tumefaciens-mediated transformation. The transformation caused a significant change in mycelial and conidial yields, and in extracellular chitinase activity in some transformants. The cultural filtrates of some transformants also decreased acetylcholinesterase activity and the survival of Heterodera filipjevi second-stage juveniles relative to the wild-type strain. One transformant (G10) had a growth rate and biocontrol efficacy similar to the wild-type strain, so it was used for a pilot study of B. bassiana colonization conducted over 13 weeks. Real-time PCR results and CFU counts revealed that the population of G10 increased quickly over the first 3 weeks, then decreased slowly over the following 4 weeks before stabilizing. In addition, the application of wild-type B. bassiana 08F04 and transformant G10 significantly reduced the number of H. filipjevi females in roots by 64.4% and 60.2%, respectively. The results of this study have practical applications for ecological, biological and functional studies of B. bassiana 08F04 and for bionematicide registration.
dc.description.sponsorshipNational Special Fund for Agro-scientific Research in the Public Interest of China [201503112]; Scientific and Technological Project of Henan Province [182102110272]; Special Funds for Research and Development of Henan Academy of Agricultural Sciences [2019CY02]
dc.description.sponsorshipThis research was financially supported by National Special Fund for Agro-scientific Research in the Public Interest of China (201503112), Scientific and Technological Project of Henan Province (182102110272) and Special Funds for Research and Development of Henan Academy of Agricultural Sciences (2019CY02).
dc.identifier.doi10.1371/journal.pone.0232770
dc.identifier.issn1932-6203
dc.identifier.issue5
dc.identifier.pmid32369513
dc.identifier.scopus2-s2.0-85084276946
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0232770
dc.identifier.urihttps://hdl.handle.net/11480/15624
dc.identifier.volume15
dc.identifier.wosWOS:000537280000046
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectReal-Time Pcr
dc.subjectTumefaciens-Mediated Transformation
dc.subjectPlant-Parasitic Nematodes
dc.subjectAgrobacterium-Tumefaciens
dc.subjectPseudomonas-Fluorescens
dc.subjectBiological-Control
dc.subjectT-Dna
dc.subjectGene
dc.subjectQuantification
dc.subjectStrain
dc.titleColonization of Beauveria bassiana 08F04 in root-zone soil and its biocontrol of cereal cyst nematode (Heterodera filipjevi)
dc.typeArticle

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