Exploring the efficacy of RNAi-mediated gene knock-down via oral delivery of dsRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say)

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dc.authoridTariq, Haneef/0000-0002-5148-3028
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridGokce, Ayhan/0000-0002-5617-1349
dc.contributor.authorTariq, Haneef
dc.contributor.authorGokce, Ayhan
dc.contributor.authorAksoy, Emre
dc.contributor.authorElci, Eminur
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:34:26Z
dc.date.available2024-11-07T13:34:26Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractRNA interference (RNAi) is a versatile genetic tool capable of selectively inhibiting the expression of any gene in a targeted organism. Its implementation holds great promise for safeguarding crops against insect pests and diseases. Vacuolar-ATPase represents an ideal target for RNAi-based pest management strategies since it is an enzyme essential for various physiological processes in insects. In this study, double-stranded RNA (dsRNA) was synthesized using an L4440 vector in Escherichia coli HT115 strain to silence the Vacuolar-ATPase proteolipid subunit mRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say). To assess the effectiveness of RNAi, L. decemlineata larvae at different developmental stages were fed potato leaflets treated with dsRNA. The feeding bioassays using dsV-ATPase resulted in significant mortality rates, ranging from 45% to 77% across all of the instar stages of L. decemlineata. Furthermore, ingestion of dsRNAs by third-and fourth-instar larvae exerted significant effects on their body weight and foliage consumption. Notably, feeding the larvae dsV-ATPase led to a significant reduction in V-ATPase gene expression, confirming the efficacy of RNAi-mediated gene silencing in controlling L. decemlineata populations. These findings highlight the potential of RNAi-mediated gene silencing as a valuable strategy for managing L. decemlineata populations by targeting essential genes.
dc.description.sponsorshipAyhan Scedil;ahenk Foundation
dc.description.sponsorshipThis study is part of the PhD thesis of HT, who is currently pursuing his PhD under the supervision of AG. We acknowledge Dr. Muneeb HASSAN HASHM & Idot; for his guidance and support during the vector construction. The authors also acknowledge the Ayhan & Scedil;ahenk Foundation for providing fellowship during the doctoral study of HT.
dc.identifier.doi10.55730/1300-0179.3150
dc.identifier.endpage504
dc.identifier.issn1300-0179
dc.identifier.issn1303-6114
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85176961622
dc.identifier.scopusqualityQ3
dc.identifier.startpage492
dc.identifier.trdizinid1208759
dc.identifier.urihttps://doi.org/10.55730/1300-0179.3150
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1208759
dc.identifier.urihttps://hdl.handle.net/11480/15963
dc.identifier.volume47
dc.identifier.wosWOS:001105431500003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Zoology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectRNA interference
dc.subjectVacuolar-ATPase
dc.subjectmortality
dc.subjecttranscript expression
dc.titleExploring the efficacy of RNAi-mediated gene knock-down via oral delivery of dsRNA in the Colorado potato beetle (Leptinotarsa decemlineata Say)
dc.typeArticle

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