Escherichia coli O157 in fish: Prevalence, antimicrobial resistance, biofilm formation capacity, and molecular characterization
Küçük Resim Yok
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study was performed to survey the incidence of Escherichia coli O157:H7 contamination in fish samples which were obtained from different fish farms and retail markets. For this purpose, a total of 140 fish samples were analyzed according to ISO 16654 and screened for virulence genes by mPCR. Antibiotic susceptibility tests were performed with the disc diffusion method and isolates were genotyped by using Enterobacterial repetitive intergenic consensus (ERIC) PCR. Of the 140 analyzed sample, two (1.4%), from the same farm, were found to be contaminated with E. coli O157 serogroup, one of which harbored stx1 and the other eaeA gene. E. coli O157 serogroup were resistant to only ciprofloxacin and were not capable of forming biofilm and their ERIC-PCR patterns were different. In conclusion, the existence of pathogenic E. coli O157 serogroup in fish samples might be a significant threat to public health and fish could serve as a vehicle for transmission of these bacteria to consumers in Turkey.
Açıklama
Anahtar Kelimeler
Antibiotic resistance, Biofilm, E. coli O157, ERIC-PCR, Fish
Kaynak
Lwt-Food Science and Technology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
133
