First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)

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dc.contributor.authorBakhsh, Allah
dc.contributor.authorAasim, Muhammad
dc.contributor.authorZia, Abu Bakir
dc.contributor.authorDoğan, Muhammet
dc.contributor.authorSadi, Gökhan
dc.contributor.authorKarataş, Mehmet
dc.contributor.authorKhawar, Khalid Mahmood
dc.date.accessioned2024-11-07T13:20:14Z
dc.date.available2024-11-07T13:20:14Z
dc.date.issued2016
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing ?-glucuronidase (GUS) and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medium containing 1.0 mg/l IBA and 100 mg/l Kanamycin where 43 plants survived and rooted. Expression of GUS gene in the putative transgenics was confirmed by histochemical GUS assay. Visible localised gus expression was noted in a few cells and callus tissues of 4 plantlets that were photographed using compound light microscope.
dc.identifier.endpage645
dc.identifier.issn2148-127X
dc.identifier.issue8
dc.identifier.startpage642
dc.identifier.trdizinid203499
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/203499
dc.identifier.urihttps://hdl.handle.net/11480/13715
dc.identifier.volume4
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241107
dc.subjectBiyoloji
dc.subjectBitki Bilimleri
dc.titleFirst Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)
dc.typeArticle

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