Thymoquinone reduces methotrexate-induced heart damage: a histopathological study in rats

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dc.authoridONAL, Esra/0000-0002-2631-6033
dc.authoridKULOGLU, NURHAN/0000-0002-1199-2784
dc.authoridAKIN, Ali Tugrul/0000-0002-1408-8571
dc.authoridYildirim, Aysegul Burcin/0000-0001-7240-9997
dc.authoridCEYLAN, TAYFUN/0000-0002-0917-0378
dc.authoridKAYMAK, EMIN/0000-0002-3818-2693
dc.contributor.authorYildirim, Aysegul Burcin
dc.contributor.authorKaymak, Emin
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorAkin, Ali Tugrul
dc.contributor.authorKuloglu, Nurhan
dc.contributor.authorSayan, Meryem
dc.contributor.authorDeger, Necla
dc.date.accessioned2024-11-07T13:35:14Z
dc.date.available2024-11-07T13:35:14Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPurpose: The study aimed to evaluate the effect of thymoquinone on cardiac tissue in MTX-induced cardiac toxicity in rats with various parameters.Materials and Methods: Group I (n=8) was administered intraperitoneal saline for 10 days. Intraperitoneal olive oil was applied to Group II (n=8) for 10 days. Group III (n=8) received 10 mg/kg Thymoquinone (THQ) intraperitoneally for 10 days. Group IV (n=8) was administered a single dose of 20 mg/kg Methotrexate (MTX), 500 mg/20 ml, intraperitoneally on the 1st day of the experiment. Group V (n=8) MTX: 20 mg/kg single dose intraperitoneally on the 1st day; THQ: 10mg/kg i.p. administered for 10 days. Since Methotrexate was in liquid form, no solvent was used. At the end of the experimental period, the rats were sacrificed for analysis of heart tissue. The structure of heart tissue was evaluated by hematoxylin-eosin staining. Immunohistochemically, Connexin-43, HSP90, and HIF- 1 alpha antibodies were stained.Results: Group IV was found to have histopathological deterioration, which was ameliorated by THQ. In addition to this; Connexin-43 immunoreactivity was the lowest in Group IV compared to other groups: 108.5 +/- 7.4. Compared to other groups, HSP90 immunoreactivity was highest in Group IV: 103.6 +/- 10.4. Compared to other groups, HIF-1 alpha immunoreactivity was highest in Group IV: 95.2 +/- 9.1.Conclusion: Thymoquinone has a positive effect on Connexin-43, one of the proteins providing conduction in intercalary discs, HSP90, one of the chaperones in the cell and HIF-1 alpha expression against MTX toxicity. At the same time, THQ provides a significant improvement in cardiac tissue histopathologically by showing a cardioprotective effect.
dc.description.sponsorshipYozgat Bozok University Scientific Research Projects (BAP) [21/036]
dc.description.sponsorshipThis study was supported by Yozgat Bozok University Scientific Research Projects (BAP) Coordinatorship with project number 21/036.
dc.identifier.doi10.17826/cumj.1314101
dc.identifier.endpage851
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue3
dc.identifier.startpage844
dc.identifier.trdizinid1199190
dc.identifier.urihttps://doi.org/10.17826/cumj.1314101
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1199190
dc.identifier.urihttps://hdl.handle.net/11480/16398
dc.identifier.volume48
dc.identifier.wosWOS:001117679900017
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherCukurova Univ, Fac Medicine
dc.relation.ispartofCukurova Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectConnexin-43
dc.subjectHSP90
dc.subjectHIF-1 alpha
dc.subjectmethotrexate
dc.subjectthymoquinone
dc.titleThymoquinone reduces methotrexate-induced heart damage: a histopathological study in rats
dc.typeArticle

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