Ectopic expression of common bean ERF transcription factorPvERF35promotes salt stress tolerance in tobacco
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the present study, a TINY-like AP2/ERF gene,PvERF35i,was amplified from common bean (Phaseolus vulgarisL.), cloned and functionally characterized by overexpressing in tobacco cv. Petite havana. Transgenic plants overexpressingPvERF35were generated usingAgrobacterium-mediated transformation and used to evaluate the possible roles of the transgene under salt stress conditions. Evaluation of transgenics was completed using both molecular and biochemical analysis. PCR, Southern blot and RT-qPCR assays revealed the correct integration and enhanced expression of the transgene. Physiological and biochemical analysis of transgenic plants showed their better performance compared to the wild type in terms of germination and survival rates and root and shoot growth under salt stress treatment (200 mM NaCl). Having a high concentration of proline, APX and POX, thePvERF35overexpressed plants were physiologically and morphologically less affected by salt stress application.In silicopromoter analysis of thePvERF35gene led to identification of importantcis-regulatory elements, MYB, MYC and TGACG-motif, annotated with salt response of plants. The protein-protein interaction network showed that there was a strong association between ABC transporter proteins and PvERF35 protein. Salt stress-related miRNA, miRNA156 and miRNA159, targetingPvERF35were identified usingin silicotarget finding analysis. These findings suggest thatPvERF35functions as a stress-responsive transcription factor in differential modulation of salt stress tolerance and may have applications in the engineering of economically important crops.
Açıklama
Anahtar Kelimeler
DREB, interaction network, miRNA, Phaseolus vulgaris, salt stress, TINY2
Kaynak
Plant Biology
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
22
Sayı
6
