Ectopic expression of common bean ERF transcription factorPvERF35promotes salt stress tolerance in tobacco

Basit Öğe Kaydı
dc.authoridsecgin, zafer/0000-0002-1952-1784
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridKAVAS, MUSA/0000-0001-5903-2873
dc.contributor.authorKavas, M.
dc.contributor.authorGokdemir, G.
dc.contributor.authorSecgin, Z.
dc.contributor.authorBakhsh, A.
dc.date.accessioned2024-11-07T13:32:19Z
dc.date.available2024-11-07T13:32:19Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractIn the present study, a TINY-like AP2/ERF gene,PvERF35i,was amplified from common bean (Phaseolus vulgarisL.), cloned and functionally characterized by overexpressing in tobacco cv. Petite havana. Transgenic plants overexpressingPvERF35were generated usingAgrobacterium-mediated transformation and used to evaluate the possible roles of the transgene under salt stress conditions. Evaluation of transgenics was completed using both molecular and biochemical analysis. PCR, Southern blot and RT-qPCR assays revealed the correct integration and enhanced expression of the transgene. Physiological and biochemical analysis of transgenic plants showed their better performance compared to the wild type in terms of germination and survival rates and root and shoot growth under salt stress treatment (200 mM NaCl). Having a high concentration of proline, APX and POX, thePvERF35overexpressed plants were physiologically and morphologically less affected by salt stress application.In silicopromoter analysis of thePvERF35gene led to identification of importantcis-regulatory elements, MYB, MYC and TGACG-motif, annotated with salt response of plants. The protein-protein interaction network showed that there was a strong association between ABC transporter proteins and PvERF35 protein. Salt stress-related miRNA, miRNA156 and miRNA159, targetingPvERF35were identified usingin silicotarget finding analysis. These findings suggest thatPvERF35functions as a stress-responsive transcription factor in differential modulation of salt stress tolerance and may have applications in the engineering of economically important crops.
dc.description.sponsorshipResearch Fund of Ondokuz Mays University [PYO.ZRT.1904.15.010]
dc.description.sponsorshipThis research was supported by Research Fund of Ondokuz Mays University PYO.ZRT.1904.15.010.
dc.identifier.doi10.1111/plb.13167
dc.identifier.endpage1112
dc.identifier.issn1435-8603
dc.identifier.issn1438-8677
dc.identifier.issue6
dc.identifier.pmid32777125
dc.identifier.scopus2-s2.0-85092891709
dc.identifier.scopusqualityQ1
dc.identifier.startpage1102
dc.identifier.urihttps://doi.org/10.1111/plb.13167
dc.identifier.urihttps://hdl.handle.net/11480/15351
dc.identifier.volume22
dc.identifier.wosWOS:000581055800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofPlant Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectDREB
dc.subjectinteraction network
dc.subjectmiRNA
dc.subjectPhaseolus vulgaris
dc.subjectsalt stress
dc.subjectTINY2
dc.titleEctopic expression of common bean ERF transcription factorPvERF35promotes salt stress tolerance in tobacco
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu