The Inhibition Effect of Curcumin on MCF-7 Breast Cancer Cells via GSK-3beta and VEGF Signals
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Date
2021
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info:eu-repo/semantics/openAccess
Abstract
Objective: Nowadays, there is a tendency towards supportive and alternative therapies in cancer treatment. So, theaim was to search the effect of curcumin on the cell viability and migration via GSK-3beta and VEGF in MCF-7breast cancer cells and L929 fibroblast cells.Materials and Methods: In the experiment, MCF-7 breast cancer cells and L929 fibroblast cells were treated withcurcumin at five concentrations (5, 10, 20, 40 and 80µM) for 24 and 48 hours, and MTT assay was used to detect thecytotoxicity level of curcumin. For immunocytochemical staining, both cells were exposed with 5µM, 20µM and80µM of curcumin for 48 hours. The immunocytochemistry method was performed to evaluate the expressions ofGSK-3beta and VEGF. The immunocytochemical results were evaluated using H-score. For cell migration, the scratchwound assay was done at three concentrations for 48 hours, and the percentage of wound closure was calculated. Alldata were analyzed statistically.Results: After MTT assay, it was observed that curcumin had a dose-dependent toxic effect on MCF-7 cancer cellscompared to L929 cells. The immunocytochemical distributions of GSK-3beta and VEGF were significantlydecreased with the curcumin treatment in MCF-7 cells. However, curcumin showed a marked inhibitory effect on thecell migration in comparison with the non-treated MCF-7 cells and L929 cells.
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Tıbbi Araştırmalar Deneysel, Onkoloji, Biyokimya ve Moleküler Biyoloji
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Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi
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Volume
8
Issue
2
