Inducing osmotic stress leads to better genetic transformation efficiency in cotton (Gossypium hirsutum L.)

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dc.authorid0000-0003-3561-7863
dc.authorid0000-0002-5547-8488
dc.contributor.authorBarpete, Surendra
dc.contributor.authorBakhsh, Allah
dc.contributor.authorAnayol, Emine
dc.contributor.authorOzcan, Sancar Fatih
dc.contributor.authorOguz, Muhammet Cagri
dc.contributor.authorKarakoc, Omer Cem
dc.contributor.authorOzcan, Sebahattin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractThe present study investigated the effect of different salts on cotton shoot regeneration and transformation efficiency. Two-day-old germinating embryos of a local cotton cultivar (SG-125) were pretreated with 50 mM each of NaCl, CaCl2, and KCl for 60 min. The embryo explants were transformed by cocultivation with Agrobacterium tumefaciens strain LBA 4404 harboring a binary plasmid pTF101.1 that carried the insecticidal gene (cry1Ac) under control of wound-inducible promoter (AoPR1) and bilanafos acetyl reductase (bar) gene for plant selection. The salt-pretreated embryos showed maximum response on regeneration MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA), also supplemented with 5 mg/L bialaphos for in vitro screening of the transformed plantlets. The primary transformants were further screened by molecular techniques for integration and expression of the introduced gene. Maximum transformation efficiency (1.10%) was noted on KCl-treated explants compared to nontreated (control) explants. In conclusion, pretreatment of explants with 50 mM KCl for 60 min induced positive effects and triggered shoot regeneration in primary cotton transformants.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [111O254]
dc.description.sponsorshipThe authors are grateful to the Scientific and Technological Research Council of Turkey (TUBITAK) for financial support of the research work (TUBITAK project no.: 111O254). The authors are also grateful to Prof Khalid Mahmood Khawar for his valuable suggestions for the improvement of the manuscript.
dc.identifier.doi10.3906/biy-1509-16
dc.identifier.endpage836
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84975296989
dc.identifier.scopusqualityQ2
dc.identifier.startpage826
dc.identifier.trdizinid214736
dc.identifier.urihttps://dx.doi.org/10.3906/biy-1509-16
dc.identifier.urihttps://hdl.handle.net/11480/3785
dc.identifier.volume40
dc.identifier.wosWOS:000378689600012
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCotton
dc.subjectin vitro
dc.subjectosmotic treatment
dc.subjectregeneration
dc.subjecttransformation
dc.titleInducing osmotic stress leads to better genetic transformation efficiency in cotton (Gossypium hirsutum L.)
dc.typeArticle

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