Immunodetection of tubulin and centromeric histone H3 (CENH3) proteins in Glycine species
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BackgroundThe centromeres appear as primary constrictions on monocentric metaphase chromosomes; where sister chromatids are held together and assemble the proteinaceous kitechore complex at which microtubule proteins attach during nuclear divisions for pulling sister chromatids to opposite cell poles. The movement of chromosomes is usually governed by structural proteins that are either species-specific or highly conserved, such as the centromere-specific histone H3 (CENH3) and tubulin proteins, respectively. Methods and resultsWe aimed to detect these proteins across eight different Glycine species by an immunofluorescence assay using specific antibodies. Furthermore, with the alpha-tubulin antibody we traced the dynamics of microtubules during the mitotic cell cycle in Glycine max. With two-color immunofluorescence staining, we showed that both proteins interact during nuclear division. ConclusionsFinally, we proved that in different diploid and tetraploid Glycine species CENH3 can be detected in functional centromeres with spatial proximity of microtubule proteins.
Açıklama
Anahtar Kelimeler
Centromeric histone H3 (CENH3), Glycine, Immunofluorescence, Soybean, Tubulin
Kaynak
Molecular Biology Reports
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
51
Sayı
1
