Immunodetection of tubulin and centromeric histone H3 (CENH3) proteins in Glycine species

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dc.authoridTek, Ahmet L./0000-0002-3292-5142
dc.contributor.authorAkkamis, Huemeyra Yildiz
dc.contributor.authorTek, Ahmet L.
dc.date.accessioned2024-11-07T13:24:18Z
dc.date.available2024-11-07T13:24:18Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackgroundThe centromeres appear as primary constrictions on monocentric metaphase chromosomes; where sister chromatids are held together and assemble the proteinaceous kitechore complex at which microtubule proteins attach during nuclear divisions for pulling sister chromatids to opposite cell poles. The movement of chromosomes is usually governed by structural proteins that are either species-specific or highly conserved, such as the centromere-specific histone H3 (CENH3) and tubulin proteins, respectively. Methods and resultsWe aimed to detect these proteins across eight different Glycine species by an immunofluorescence assay using specific antibodies. Furthermore, with the alpha-tubulin antibody we traced the dynamics of microtubules during the mitotic cell cycle in Glycine max. With two-color immunofluorescence staining, we showed that both proteins interact during nuclear division. ConclusionsFinally, we proved that in different diploid and tetraploid Glycine species CENH3 can be detected in functional centromeres with spatial proximity of microtubule proteins.
dc.description.sponsorshipTurkish Council of Higher Education, Trkiye
dc.description.sponsorshipWe thank Prof. Ingo Schubert (IPK, Germany) for his critical reading and valuable suggestions on the manuscript. We thank the anonymous reviewers for their contributions.
dc.identifier.doi10.1007/s11033-024-09730-z
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1
dc.identifier.pmid39001981
dc.identifier.scopus2-s2.0-85198372553
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11033-024-09730-z
dc.identifier.urihttps://hdl.handle.net/11480/14043
dc.identifier.volume51
dc.identifier.wosWOS:001272460200009
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectCentromeric histone H3 (CENH3)
dc.subjectGlycine
dc.subjectImmunofluorescence
dc.subjectSoybean
dc.subjectTubulin
dc.titleImmunodetection of tubulin and centromeric histone H3 (CENH3) proteins in Glycine species
dc.typeArticle

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