Genetically Engineered Ricin Suppresses Bactrocera dorsalis (Diptera: Tephritidae) based on Demographic Analysis of Group-Reared Life Table

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dc.contributor.authorChang, Cheng
dc.contributor.authorHuang, Chun-Yen
dc.contributor.authorDai, Shu-Mei
dc.contributor.authorAtlihan, Remzi
dc.contributor.authorChi, Hsin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractThe oriental fruit fly, Bactrocera dorsalis (Hendel), reduces the quantity and quality of many host fruits through the process of oviposition and larval feeding, and this insect has been considered a major insect pest in several Asian countries for decades. Using an earlier-developed, female-specific system that combines the toxicity of the ricin A chain (RTA) and the alternative RNA splicing property of doublesex (Bddsx), we show that transgenic male flies harboring the RTA-Bddsx transgene unevenly repress the pest population through inheritable effects. In age-stage, two-sex life-table analyses, high larval mortality and a delay in pupation were observed after introducing the transgene. The high male to female ratio in DsRed(+) flies demonstrates the lethal effect of ricin on females. The fitness of both the DsRed(+)- and DsRed(-)-transformed females was reduced as shown in the decrease of the net reproductive rate (R-0), intrinsic rate (r), and finite rate (lambda) values compared with the wild-type populations. The integrity of the RTA-Bddsx transgene remained in more than 80% of DsRed(+) males after ten generations, supporting the stable inheritance of the transgene. All of the data from this study support the proposed RTA-Bddsx SIT approach, which provides a species-specific and environmentally friendly method of suppressing, rather than eradiating, B. dorsalis.
dc.description.sponsorshipMinistry of Science and Technology, Taiwan [MOST102-2321-B-005-007, MOST103-2321-B-005-007]
dc.description.sponsorshipWe would like to acknowledge the contributions of the reviewers and editor for their valuable comments and suggestions, all of which were instrumental in improving this article. This study was partly supported by grants (MOST102-2321-B-005-007, MOST103-2321-B-005-007) from the Ministry of Science and Technology, Taiwan. In addition, we would like to thank the ATU plan of the Ministry of Education for publication support.
dc.identifier.doi10.1093/jee/tow091
dc.identifier.endpage992
dc.identifier.issn0022-0493
dc.identifier.issn1938-291X
dc.identifier.issue3
dc.identifier.pmid27122495
dc.identifier.scopus2-s2.0-85008199345
dc.identifier.scopusqualityQ1
dc.identifier.startpage987
dc.identifier.urihttps://dx.doi.org/10.1093/jee/tow091
dc.identifier.urihttps://hdl.handle.net/11480/3638
dc.identifier.volume109
dc.identifier.wosWOS:000378041400001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherOXFORD UNIV PRESS INC
dc.relation.ispartofJOURNAL OF ECONOMIC ENTOMOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectdoublesex gene of Bactrocera dorsalis
dc.subjectricin A chain
dc.subjectage-stage
dc.subjecttwo-sex life table
dc.subjectsterile insect technique
dc.titleGenetically Engineered Ricin Suppresses Bactrocera dorsalis (Diptera: Tephritidae) based on Demographic Analysis of Group-Reared Life Table
dc.typeArticle

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