Genetically Engineered Ricin Suppresses Bactrocera dorsalis (Diptera: Tephritidae) based on Demographic Analysis of Group-Reared Life Table
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
OXFORD UNIV PRESS INC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The oriental fruit fly, Bactrocera dorsalis (Hendel), reduces the quantity and quality of many host fruits through the process of oviposition and larval feeding, and this insect has been considered a major insect pest in several Asian countries for decades. Using an earlier-developed, female-specific system that combines the toxicity of the ricin A chain (RTA) and the alternative RNA splicing property of doublesex (Bddsx), we show that transgenic male flies harboring the RTA-Bddsx transgene unevenly repress the pest population through inheritable effects. In age-stage, two-sex life-table analyses, high larval mortality and a delay in pupation were observed after introducing the transgene. The high male to female ratio in DsRed(+) flies demonstrates the lethal effect of ricin on females. The fitness of both the DsRed(+)- and DsRed(-)-transformed females was reduced as shown in the decrease of the net reproductive rate (R-0), intrinsic rate (r), and finite rate (lambda) values compared with the wild-type populations. The integrity of the RTA-Bddsx transgene remained in more than 80% of DsRed(+) males after ten generations, supporting the stable inheritance of the transgene. All of the data from this study support the proposed RTA-Bddsx SIT approach, which provides a species-specific and environmentally friendly method of suppressing, rather than eradiating, B. dorsalis.
Açıklama
Anahtar Kelimeler
doublesex gene of Bactrocera dorsalis, ricin A chain, age-stage, two-sex life table, sterile insect technique
Kaynak
JOURNAL OF ECONOMIC ENTOMOLOGY
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
109
Sayı
3
