Antioxidant and Antigenotoxic Potential of Infundibulicybe geotropa Mushroom Collected from Northwestern Turkey
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi Ltd
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Infundibulicybe geotropa (Bull.) Harmaja is an edible mushroom found in Bolu province in northwestern Turkey. The chemical composition and bioactivity of these mushrooms has not been previously investigated. We examined the phenolic composition, elemental content, and antioxidant and antigenotoxic effects of methanol extracts of fruiting bodies. The phenolic compounds in the fungal samples were determined using high-performance liquid chromatography (HPLC), and element content was determined using atomic absorption spectrophotometry. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined using the commercially available Rel assay kit. The antigenotoxic effects of the extract were determined using the MTT assay to assess cell viability and the alkaline single-cell gel electrophoresis assay (Comet assay). The total phenolic content (ppm) of I. geotropa was found to be catechin (361 +/- 2.31), clorogenic acid (553.54 +/- 5.06), and coumaric acid (9.93 +/- 0.25). The TAS, TOS, and OSI of the extract were 1.854 +/- 0.051 mmol/L, 30.385 +/- 0.399 mu mol/L, and 1.639 +/- 0.067, respectively. The elemental levels were within normal range. In HT22 mouse hippocampal neuronal cells, the extract (100 and 200 mu g/ml) showed no genotoxic potential and ameliorated hydrogen peroxide- (H2O2-) induced oxidative DNA damage. I. geotropa may be considered a good nutrient due to its phenolic constituents and antioxidant potential.
Description
Keywords
Culinary-Medicinal Mushrooms, Radical Scavenging Activity, Age-Related Diseases, 3 Edible Mushrooms, Oxidative Stress, Chemical-Composition, Volatile Composition, Metal Concentration, Health-Benefits, Extracts
Journal or Series
Oxidative Medicine and Cellular Longevity
WoS Q Value
Q2
Scopus Q Value
Q1
Volume
2020
