Expression of thermostable alpha-amylase gene from Bacillus stearothermophilus in various Bacillus subtilis strains
Küçük Resim Yok
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The recombinant plasmid pC194Amy harbouring 5.25 kbp DNA fragment encoding the thermostable of-amylase gene of Bacillus stearothermophilus was introduced into Bacillus subtilis YB886 and B. subtilis BR151 strains by electroporation. The expressing of the alpha-amylase gene in recombinant strains were confirmed on starch plate, SDS-PAGE and zymogram analysis. In the condition of 100 degrees C and 15 min, Y13886Amy, BR151Amy and ATCC31786 alpha-amylases were maintained up to 80, 67 and 52% of their maximum activity respectively. The specific enzyme activities of YB886Amy, BR151Amy and ATCC 31786 were estimated as 2.59, 2.98 and 2.9 mu M/mg protein/min respectively.
Açıklama
Anahtar Kelimeler
Bacillus stearothermophilus, thermostable alpha-amylase, Bacillus subtilis, electroporation
Kaynak
ANNALS OF MICROBIOLOGY
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
58
Sayı
2
