Expression of thermostable alpha-amylase gene from Bacillus stearothermophilus in various Bacillus subtilis strains

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dc.authorid0000-0002-9198-656X
dc.contributor.authorOzcan, Bahri Devrim
dc.contributor.authorOzcan, Numan
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2008
dc.departmentNiğde ÖHÜ
dc.description.abstractThe recombinant plasmid pC194Amy harbouring 5.25 kbp DNA fragment encoding the thermostable of-amylase gene of Bacillus stearothermophilus was introduced into Bacillus subtilis YB886 and B. subtilis BR151 strains by electroporation. The expressing of the alpha-amylase gene in recombinant strains were confirmed on starch plate, SDS-PAGE and zymogram analysis. In the condition of 100 degrees C and 15 min, Y13886Amy, BR151Amy and ATCC31786 alpha-amylases were maintained up to 80, 67 and 52% of their maximum activity respectively. The specific enzyme activities of YB886Amy, BR151Amy and ATCC 31786 were estimated as 2.59, 2.98 and 2.9 mu M/mg protein/min respectively.
dc.identifier.endpage268
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issue2
dc.identifier.startpage265
dc.identifier.urihttps://hdl.handle.net/11480/5290
dc.identifier.volume58
dc.identifier.wosWOS:000257733800015
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofANNALS OF MICROBIOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacillus stearothermophilus
dc.subjectthermostable alpha-amylase
dc.subjectBacillus subtilis
dc.subjectelectroporation
dc.titleExpression of thermostable alpha-amylase gene from Bacillus stearothermophilus in various Bacillus subtilis strains
dc.typeArticle

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