Effects of alcohol during secondary neurulation in chick embryos
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Alkol, zararları iyi bilinmesine rağmen, tüketilmeye devam edilmektedir. Alkol tüketiminin en yaygın hasarlarından biri kraniofasial anomaliler, kardiyak anomaliler ve nöral tüp kusurlarıyla karakterize fetal alkol sendromudur. Bu nedenle, alkole bağlı toksisitenin altında yatan, zaman ve doza bağlı olarak ortaya çıkan moleküler mekanizmaları anlamak çok önemlidir. Alkolün etkilerini anlamak için, yapılan çalışmaların çoğu erken nörilasyon üzerine yapılmıştır. Ancak, geç nörilasyon üzerindeki etkileri halen bilinmemektedir. Bu nedenle bu çalışmada, civciv embriyolarında alkolün sekonder nörilasyon üzerindeki etkileri araştırılmıştır. Yöntem: Leghorn cinsi embriyonik tavuk yumurtalarını kullanıldı. Kuluçka işleminin 50. saatinde, 100 uL %50 etanol çözeltisi enjekte edildi. Alkole maruz kalma süresine bağlı olarak, E3, E7 ve E10 günlerinde değişik derecelerde patolojik bozukluk belirlendi. Bulgular: Gelişim geriliği, yapısal anomaliler, kalp ve yüzdeki morfolojik anomaliler ve özellikle iki spinal kord boşluğunun varlığı saptandı. Buna ek olarak, nöral tüpün kapanmasında gecikmeler, hücresel deformasyonlar ve notokordun oluşumunda anomaliler belirlendi. ENOS, iNOS ve TUNEL düzeyleri artarken, laminin önemli ölçüde azaldığı görüldü. Sonuç: Bu çalışmada geç gelişme döneminde alkolün yol açtığı morfolojik ve histopatolojik değişiklikler gözlendi. Alkolden kaynaklanan oksidatif stres düzeyindeki artışa matriks kompozisyonundaki değişiklikler eşlik etti. Hücre davranışını etkileyen bu mekanizmaların daha iyi anlaşılması önemli olup, alkolün zararlı etkilerinin öğrenilmesine izin verecektir.
Objective: Alcohol continues to be consumed even though its harmful effecs are well established. One of the most common damage of alcohol consumption is fetal alcohol syndrome, characterized by craniofacial anomalies, cardiac anomalies and neural tube defects. Therefore, understanding the molecular mechanisms underlying the alcohol-induced toxicity that occur with time and dose dependent manner is very important. , Most of the studies in order to understand the effects of alcohol have been carried out on early neurulation, however its effects on late neurulation are still unknown. Therefore in this study, effects of alcohol on secondary neurulation were investigated in chick embryos.Methods: Leghorn breed of embryonic chicken eggs were used. At 50 h of incubation, 100 ?L 50% ethanol solution was injected. Depending on the period of exposure to alcohol, varying degrees of pathological disorders were detected in E3, E7 and E10 days. Results: Developmental delay, structural abnormalities, morphological abnormalities in the heart and face and especially presence of two spinal cord cavities were found. In addition, we also detected delays in the closure of the neural tube, cellular deformities and the structural abnormalities in notochord. While eNOS, iNOS, and TUNEL levels increased, while laminin levels decreased.Conclusion: In this study during late development, significant alcohol-induced morphological and histopathological changes were observed. We also determined Increased level of oxidative stress caused by alcohol was accompanied with the changes in matrix composition. Better understanding of these mechanisms which affect the cell behavior is important and will allow learning of harmful effects of alcohol.
Objective: Alcohol continues to be consumed even though its harmful effecs are well established. One of the most common damage of alcohol consumption is fetal alcohol syndrome, characterized by craniofacial anomalies, cardiac anomalies and neural tube defects. Therefore, understanding the molecular mechanisms underlying the alcohol-induced toxicity that occur with time and dose dependent manner is very important. , Most of the studies in order to understand the effects of alcohol have been carried out on early neurulation, however its effects on late neurulation are still unknown. Therefore in this study, effects of alcohol on secondary neurulation were investigated in chick embryos.Methods: Leghorn breed of embryonic chicken eggs were used. At 50 h of incubation, 100 ?L 50% ethanol solution was injected. Depending on the period of exposure to alcohol, varying degrees of pathological disorders were detected in E3, E7 and E10 days. Results: Developmental delay, structural abnormalities, morphological abnormalities in the heart and face and especially presence of two spinal cord cavities were found. In addition, we also detected delays in the closure of the neural tube, cellular deformities and the structural abnormalities in notochord. While eNOS, iNOS, and TUNEL levels increased, while laminin levels decreased.Conclusion: In this study during late development, significant alcohol-induced morphological and histopathological changes were observed. We also determined Increased level of oxidative stress caused by alcohol was accompanied with the changes in matrix composition. Better understanding of these mechanisms which affect the cell behavior is important and will allow learning of harmful effects of alcohol.
Açıklama
Anahtar Kelimeler
Pediatri
Kaynak
İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
7
Sayı
3
