Differential expression of soluble pyrophosphatase isoforms in Arabidopsis upon external stimuli

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dc.contributor.authorOeztuerk, Zahide Neslihan
dc.contributor.authorGreiner, Steffen
dc.contributor.authorRausch, Thomas
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2015
dc.departmentNiğde ÖHÜ
dc.description.abstractIn plants, pyrophosphate (PPi), generated in a wide range of reversible anabolic reactions, is hydrolyzed by pyrophosphatases. The presence of tonoplast-and Golgi-integral H+-translocating pyrophosphatases in plants led to the conclusion that plant cytosol has no soluble pyrophosphatase activity. However, the Arabidopsis thaliana (L.) Heynh. genome also encodes five soluble pyrophosphatase isoforms (PPas). There are almost no data in the literature on their redundancy in plant metabolism; therefore, we performed expression analyses of A. thaliana soluble pyrophosphatase isoforms in response to external stimuli including carbohydrate status, hormone action, and stress exposure. The results revealed pronounced specificity for each isoform. Interestingly, one isoform (PPa3; At2g46860) was specifically induced during seedling etiolation, and in the presence of the nonmetabolizable sugar 3-O-methylglucose. Based on quantitative PCR analyses, PPa1 (At1g01050) and PPa4 (At3g53620) appeared to be regulated by sugars. Quantitative PCR analyses indicated isoform-and tissue-dependent responses of PPa isoforms to ABA, salt, and cold stresses. PPa2 (At2g18230) and PPa5 (At4g01480) responded differentially to salinity, cold treatment, and ABA. We conclude that plant cytosolic pyrophosphatases perform multiple, so far overlooked functions during plant development and stress exposure.
dc.description.sponsorshipKWS SAAT AG; Sudzucker AG
dc.description.sponsorshipThe authors would like to acknowledge KWS SAAT AG and Sudzucker AG for their financial support. We further thank Sebastian Wolf, Jan Eufinger, Andreas Wachter, Roland Gromes, and Slobodanka Grsic for their valuable discussions and Emilia Sancho-Vargas and Heike Steininger for their technical assistance.
dc.identifier.doi10.3906/bot-1405-70
dc.identifier.endpage579
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84934297459
dc.identifier.scopusqualityQ2
dc.identifier.startpage571
dc.identifier.trdizinid174071
dc.identifier.urihttps://dx.doi.org/10.3906/bot-1405-70
dc.identifier.urihttps://hdl.handle.net/11480/4040
dc.identifier.volume39
dc.identifier.wosWOS:000357585000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BOTANY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytosolic pyrophosphatase
dc.subjectArabidopsis thaliana (L.) Heynh.
dc.subjectsalt stress
dc.subjectcold stress
dc.subjectABA response
dc.subjectphosphate starvation
dc.subjectcarbohydrate regulation
dc.subjectetiolation
dc.titleDifferential expression of soluble pyrophosphatase isoforms in Arabidopsis upon external stimuli
dc.typeArticle

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