Boric acid alleviates periodontal inflammation induced by IL-1? in human gingival fibroblasts

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dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorHakki, Sema S.
dc.contributor.authorNielsen, Forrest H.
dc.date.accessioned2024-11-07T13:32:55Z
dc.date.available2024-11-07T13:32:55Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: Boric acid (BA) has been found to have therapeutic effects on periodontal disease through beneficially affecting antibacterial, anti-viral, and anti-inflammatory actions. Methods: This study was conducted to determine the effect of BA on cell viability and on mRNA expressions of proinflammatory and anti-inflammatory cytokines and on oxidative stress enzymes induced by IL-1 beta (1 ng/mL) in Human Gingival Fibroblasts (HGF) cultured for 24 and 72 h in DMEM media. The BA concentrations added to the media were 0.09 %, 0.18 %, 0.37 %, and 0.75 %. Results: All of the BA concentrations increased the viability of cell cultured in DMEM media only, indicating that these concentrations were not toxic and actually beneficial to cell viability. The addition of 1 ng/m: of IL-1 beta decreased cell viability that was overcome by all concentrations of BA at both 24 and 72 h. The IL-1 beta addition to the media increased the expressions of the proinflammatory cytokines IL-1 beta, IL-6, IL-8, and IL-17; the antiinflammatory cytokine IL-10; and the oxidative stress enzymes superoxide dismutase (SOD0 and glutathione peroxidase (GPX). The IL-1 beta induced increase mRNA expression of IL-1 beta was decreased at 24 h by the 0.37 % and 0.75 % BA additions to the media and decreased in a dose-dependent manner by all concentrations of BA at 72 h. The IL-1 beta induced increase in the expression of IL-6 was decreased in dose-dependent manner at 72 h by BA. All BA concentrations decreased the IL-1 beta induced expression of IL-8 at both 24 and 72 h. The induced increase in IL17 by IL-1 beta was not significantly affected by the BA additions. The increase in the anti-inflammatory cytokine IL10 induced by IL-1 beta was increased further by all BA additions in dose dependent manner at both 24 and 72 h. The mRNA expressions of SOD and GPX increased by IL-1 beta were further increased by the 0.37 % and 0.75 % BA concentrations at 72 h. Conclusions: These findings indicate that BA can significantly modulate the cytokines that are involved in inflammatory stress and reactive oxygen species action and thus could be an effective therapeutic agent in the treatment of periodontal disease.
dc.description.sponsorshipThis work was not supported by any funding
dc.identifier.doi10.1016/j.jtemb.2024.127466
dc.identifier.issn0946-672X
dc.identifier.issn1878-3252
dc.identifier.pmid38692230
dc.identifier.scopus2-s2.0-85191768517
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jtemb.2024.127466
dc.identifier.urihttps://hdl.handle.net/11480/15670
dc.identifier.volume84
dc.identifier.wosWOS:001290966400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Gmbh
dc.relation.ispartofJournal of Trace Elements in Medicine and Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectBoric acid
dc.subjectPeriodontitis
dc.subjectCytokine
dc.subjectFibroblast
dc.subjectGingiva
dc.titleBoric acid alleviates periodontal inflammation induced by IL-1? in human gingival fibroblasts
dc.typeArticle

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