Identification of transcription factors and their potential roles in regulation of allelochemicals' biosynthesis in lithospermum erythrorhizon
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Niğde Ömer Halisdemir Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Alkannin/shikonin (A/S) olarak bilinen ikincil metabolitlerin (SM) üretimini yaptığı bilinen bazı bitki türleri Boraginaceae familyasının. Lithospermeae tribe üyesidir. Bu tribe içerinde yer alan Lithospermum erythrorhizon'da A/S biyosentezinin düzenlenmesinde rol oynayan MYB genleri (Miyeloblastoz viral onkogeni) henüz kapsamlı bir şekilde araştırılmamıştır. Bu çalışmada, diğer bitki türlerinde SM'lerin biyosentezini düzenlediği bulunan MYB transkripsiyon faktörleri (TF'ler) ailesini araştırmaya odaklandık. L. erythrorhizon genomunda kodlanmış en az 128 potansiyel MYB TF tanımlanmıştır. Filogenetik analiz bunları 21 alt gruba ayırmıştır. Ayrıca, 140 SlMYB ve 168 AtMYB ile birlikte 128 LeMYB'nin karşılaştırmalı filogenetik analizi sonucu 27 alt aile belirlenmiştir. Ekspresyon analizi, farklı A/S üreten koşullarda farklı sayıda MYB geninin önemli ölçüde eksprese edildiğini (FDR < 0.05) göstermiştir. Sonuç olarak, LeMYB genlerinin kapsamlı analizi ile elde edilen bulgular, familyanın diğer üyelerinin MYB protein ailesi fonksiyon çalışmalarında yardımcı olacaktır.
Lithospermeae tribe in the Boraginaceae family contains several plant species which are well recognized for the production of secondary metabolites (SMs) known as alkannin/shikonin (A/S). Myeloblastosis viral oncogenes (MYBs) involved in the regulation of A/S biosynthesis in Lithospermum erythrorhizon have not been yet comprehensively studied. In the present study, we focused on the MYB-family of transcription factors (TFs) that have been identified to regulate SMs biosynthesis in other plant species. A comprehensive genome-wide analysis identified at least 128 potential MYB TFs encoded in L. erythrorhizon genome. Phylogenetic analysis clustered them into 21 subgroups. Furthermore, a comparative phylogenetic analysis of 128 LeMYBs along with 140 SlMYBs and 168 AtMYBs indicated 27 subfamilies. Expression analysis of LeMYBs indicated that different number of MYBs are significantly expressed (FDR < 0.05) in different A/S producing conditions. Collectively, our comprehensive analysis of LeMYB genes will be helpful in future functional studies of MYB proteins family in other Lithospermeae plant species as well.
Lithospermeae tribe in the Boraginaceae family contains several plant species which are well recognized for the production of secondary metabolites (SMs) known as alkannin/shikonin (A/S). Myeloblastosis viral oncogenes (MYBs) involved in the regulation of A/S biosynthesis in Lithospermum erythrorhizon have not been yet comprehensively studied. In the present study, we focused on the MYB-family of transcription factors (TFs) that have been identified to regulate SMs biosynthesis in other plant species. A comprehensive genome-wide analysis identified at least 128 potential MYB TFs encoded in L. erythrorhizon genome. Phylogenetic analysis clustered them into 21 subgroups. Furthermore, a comparative phylogenetic analysis of 128 LeMYBs along with 140 SlMYBs and 168 AtMYBs indicated 27 subfamilies. Expression analysis of LeMYBs indicated that different number of MYBs are significantly expressed (FDR < 0.05) in different A/S producing conditions. Collectively, our comprehensive analysis of LeMYB genes will be helpful in future functional studies of MYB proteins family in other Lithospermeae plant species as well.
Açıklama
Fen Bilimleri Enstitüsü, Bitkisel Üretim ve Teknolojileri Ana Bilim Dalı
Anahtar Kelimeler
Biyoloji, Biology, Ziraat
