Microsatellite-based characterization of cotton genotypes for verticillium wilt and fiber quality traits

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dc.contributor.authorSaeed A.
dc.contributor.authorElçi E.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractCotton (Gossypium hirsutum L.) is the most important natural textile fiber crop grown worldwide. Several biotic and abiotic stress factors affect cotton yield due to lower genetic diversity for the traits of particular interest. Verticillium wilt (VW) is one of the major factors incurring huge cotton yield losses. The most effective management option against VW is the development of resistant cultivars. The resistant cultivars must also have superior fiber quality and yield traits. Therefore, the current study was aimed at screening some of the identified simple sequence repeats (SSR) markers for VW resistance and fiber quality traits of cotton genotypes in Turkey. Fifty different cultivars were screened with 30 SSR markers. Polymerase chain reaction (PCR) was conducted to amplify the SSR markers. The amplified bands were scored as 0 or 1 for absence and presence, respectively. For the molecular data analysis, polymorphism information content (PIC) values of molecular markers were calculated. Among tested SSR markers 13 were found polymorphic, which produced a total of 677 loci. The number of alleles per marker ranged from 1 to 4 and, overall average PIC values of markers ranged from 0.00 to 0.76, respectively. Principal component analysis executed on presence absence data yielded two distinct groups of cultivars screened. Hierarchical clustering revealed low genetic diversity among the tested cultivars. Based on the results TamcotCamdES, Gloria, Natalia, Lydia, Teks, Tamcot SP37H, N87 and BA525 are the promising cultivars for further breeding studies. The results of the current study also revealed that 4 SSR markers (DPL752 and DPL322 for fiber traits, DPL0022 and GH215 for VW resistance) out of 30 could be used for improving VW resistance and fiber quality in cotton through marker assisted selection. © 2018 Turkish Biochemistry Society. All rights reserved.
dc.description.sponsorshipAcknowledgment: The current study is part of Master’s Thesis completed by Amna Saeed, which has been partially supported by the Scientific and Technological Council of Turkey (TÜBİTAK) with a project number 214O086 Funder Id: 10.13039/501100004410.
dc.identifier.doi10.1515/tjb-2017-0169
dc.identifier.endpage288
dc.identifier.issn0250-4685
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85037827572
dc.identifier.scopusqualityQ4
dc.identifier.startpage277
dc.identifier.trdizinid309294
dc.identifier.urihttps://dx.doi.org/10.1515/tjb-2017-0169
dc.identifier.urihttps://hdl.handle.net/11480/1644
dc.identifier.volume43
dc.identifier.wosWOS:000435587600008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTurkish Biochemistry Society
dc.relation.ispartofTurkish Journal of Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFiber quality
dc.subjectGenetic diversity
dc.subjectGossypium hirsutum
dc.subjectSSR
dc.subjectVerticillium wilt
dc.titleMicrosatellite-based characterization of cotton genotypes for verticillium wilt and fiber quality traits
dc.typeArticle

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