Identification and expression profiling of toxic boron-responsive microRNAs and their targets in sensitive and tolerant wheat cultivars

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dc.contributor.authorKayihan, Doga Selin
dc.contributor.authorAksoy, Emre
dc.contributor.authorKayihan, Ceyhun
dc.date.accessioned2024-11-07T13:32:28Z
dc.date.available2024-11-07T13:32:28Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPost transcriptional gene regulation in wheat cultivars caused by boron (B) toxicity has not been reported to date. In this study, two wheat cultivars Bolal-2973 (B-tolerant) and Atay-85 (B-sensitive) were compared with regard to the transcriptional regulation of miR319, miR172 and miR398 and their plausible target genes in order to address differences in their performances under high B levels. The expression levels of Cu/ZnSOD gene were found to be greater in Atay exposed to toxic B compared to Bolal, as verified by stable expression level of miR398. In wheat cultivars, both toxic B concentrations might cause an induction of leaf senescence mechanism due to stable level of JA and ethylene-related miRNAs, miR172 and miR319. miR172 targeting transcription factor TOE1 was only induced under B toxicity in sensitive cultivar Atay. However, MY133 as target of miR319 was significantly upregulated under toxic B in both cultivars. Also, computational prediction of these miRNA targets in wheat was performed and their orthologs in Arabidopsis thaliana were determined. Functional protein association networks of proteins encoded by miRNA targets and gene ontology enrichment analyses of these genes were identified. We identified new sets of genes that are targets of miR172, miR319 and miR398 in T. aestivum. In addition, miR172, miR319 and miR398 are responsive to different nutrient deficiencies or toxicities such as Fe, P, B, S and Cu, suggesting crosstalk between the post-transcriptional regulatory mechanisms involving miRNAs in plants.
dc.identifier.doi10.3906/tar-2102-5
dc.identifier.endpage433
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85113716372
dc.identifier.scopusqualityQ2
dc.identifier.startpage411
dc.identifier.trdizinid514549
dc.identifier.urihttps://doi.org/10.3906/tar-2102-5
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/514549
dc.identifier.urihttps://hdl.handle.net/11480/15441
dc.identifier.volume45
dc.identifier.wosWOS:000686227800004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectArabidopsis thaliana
dc.subjectboron toxicity
dc.subjectmicroRNA
dc.subjectTriticum aestivum
dc.subjectpost-transcriptional regulation
dc.titleIdentification and expression profiling of toxic boron-responsive microRNAs and their targets in sensitive and tolerant wheat cultivars
dc.typeArticle

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