Expression levels of BAP1, OGT, and YY1 genes in patients with eyelid tumors

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Date

2021

Journal Title

Journal ISSN

Volume Title

Publisher

Walter De Gruyter Gmbh

Access Rights

info:eu-repo/semantics/openAccess

Abstract

Objectives The aim of this study was to investigate BAP1, OGT and YY1 genes and protein levels in 12 samples (8 males, 4 females) of eyelid tumor tissue with basal cell carcinoma (BCC) and 12 normal control subjects (8 males, 4 females). Methods The expression levels of these genes were determined with RT-PCR and the protein levels and expression using ELISA and IHC methods, respectively. Results In RT-PCR analysis, statistically significant upregulated expression was determined of 1.84-fold of BAP1, 2.85-fold of OGT and 3.06-fold of YY1 genes (p < 0.05). In the patient group, compared to the control group, there was a similar statistically significant strong correlation between the proteins (BAP1 and YY1; r = 0.850, BAP1 and OGT; r = 0.811, OGT and YY1; r = 0.755) (p < 0.05). In the ELISA and IHC analysis methods, a significant increase in BAP1 and YY1 protein expression levels was observed compared to the control group (p < 0.05). Conclusions The study results demonstrated that BAP1 and YY1 genes and protein levels were upregulated in eyelid tumor tissue with BCC.

Description

Keywords

BAP1, basal cell carcinoma, expression, eyelid, OGT, tumor, YY1

Journal or Series

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi

WoS Q Value

Q4

Scopus Q Value

Q4

Volume

46

Issue

6

Citation