CRISPR/Cas-mediated plant genome editing outstanding challenges a decade after implementation
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Cell Press
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.
Açıklama
Anahtar Kelimeler
Quantitative Trait Variation, De-Novo Domestication, Functional-Analysis, Crop, Tool, Mutagenesis, Expression, Maize, Overexpression, Improvement
Kaynak
Trends in Plant Science
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
28
Sayı
10
