Protein ion-exchange chromatography on a biomacromolecule-immobilized monolithic cryogel

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dc.contributor.authorÖzkan A.E.
dc.contributor.authorGüven I.
dc.contributor.authorGezici O.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractAn efficient and inexpensive monolithic stationary phase (PHEMA-HA) has been prepared through an easy process comprising addition of humic acid (HA) to a mixture of 2-hydroxyethyl methacrylate (HEMA) and N,N’-methylenebisacrylamide (MBAAm) and subsequent radical-polymerization at –20?C. The prepared monolithic material was characterized in terms of various techniques and methods such as elemental analysis, FTIR spectroscopy, scanning electron microscopy, mercury porosimetry, hydrolytic stability tests, pHpzc measurements, water-holding capacity, and water permeability. The amount of HA incorporated into the structure was calculated as 45 mg/g from the elemental analysis results. The study was conceptualized on the basis of protein ion-exchange chromatography, and some model proteins (i.e. a-chymotrypsinogen a, cytochrome c, lysozyme, human serum albumin, and myoglobin) were used in the chromatographic experiments. The effect of ionic strength and pH (5.0, 6.0, 7.0) on the retention behavior of proteins was investigated. The results revealed a typical ion-exchange behavior for the PHEMA-HA stationary phase, and with increasing gradient slope the model proteins were found to elute faster. Some model proteins could be separated by applying gradient elution where NaCl was used as the modifier. Dynamic adsorption capacity of PHEMA-HA was obtained by frontal analysis and was found as 4 mg/mL for Lys. © TÜBITAK.
dc.identifier.doi10.3906/kim-1612-65
dc.identifier.endpage370
dc.identifier.issn1300-0527
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85048008609
dc.identifier.scopusqualityQ3
dc.identifier.startpage355
dc.identifier.trdizinid531151
dc.identifier.urihttps://dx.doi.org/10.3906/kim-1612-65
dc.identifier.urihttps://hdl.handle.net/11480/1714
dc.identifier.volume42
dc.identifier.wosWOS:000431245300013
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTUBITAK
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCation exchange
dc.subjectCryogel
dc.subjectHigh-performance liquid chromatography
dc.subjectHumic acid
dc.subjectSeparation
dc.titleProtein ion-exchange chromatography on a biomacromolecule-immobilized monolithic cryogel
dc.typeArticle

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