Manipulating genome of diploid potato inbred line Solanum chacoense M6 using selectable marker gene

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dc.authoridDangol, PhD, Sarbesh Das/0000-0002-0165-6525
dc.authoridCALISKAN, MEHMET EMIN/0000-0002-4703-8853
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorDas Dangol, Sarbesh
dc.contributor.authorYel, Ilknur
dc.contributor.authorCaliskan, Mehmet Emin
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:24:35Z
dc.date.available2024-11-07T13:24:35Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe development of transgenic potatoes is imperative to investigate various gene functions as well as to develop robust potato varieties resistant to different biotic and abiotic stresses. Directing a potato breeding program from cultivated tetraploids to self-compatible diploid lines will be highly helpful for potato breeders. However, diploid potatoes are considered recalcitrant to regeneration. We aimed to develop a protocol for the transformation of diploid Solanum chacoense M6 potatoes using leaf, internodes, and microtubers as the explants. Agrobacterium tumefaciens strain GV2260 harboring pIBIN19 expression vector containing gusA gene (interrupted by an intron to induce expression from eukaryotic cells) was used for this purpose. Different inoculation times (10, 20, 30 min) were applied in aforementioned explants for gene transfer. After cocultivation, explants were transferred to the media with various hormone concentrations [6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), trans-zeatin, kinetin, and 2, 4-Dichlorophenoxyacetic acid (2,4-D)]. The calli generated were then transferred to shoot generating medium supplemented with thidiazuron (TDZ) and gibberellic acid (GA(3)). According to histochemical GUS analysis, we found a 20 min inoculation time to be optimal for gene transfer and the medium containing 2 mg L-1 BAP and 2 mg L-1 NAA was the most suitable medium for callus induction from 20 min inoculated explants (41% callus formation for internodes and 65% for leaf explants). Abundant transcripts levels of gusA gene was found in primary transfonnants when subjected to RTAPCR. GUS fluorometric assay further confirmed the primary transfonnants at protein level. The present study can serve as a gateway to transfer gene(s) of interest in diploid potatoes.
dc.description.sponsorshipGraduate Scholarship Programme for International Students [2215]; TUBITAK
dc.description.sponsorshipThe first author is a TUBITAK fellow awarded with the Graduate Scholarship Programme for International Students-2215 (PhD). Therefore, the support of TUBITAK is highly acknowledged. We are very grateful to Dr. Abdellah Barakate and Dr. Csaba Hornyik for their generous willingness to provide us diploid inbred line S. chacoense M6 in vitro plantlets from the James Hutton Institute, Dundee, UK. Authors are also thankful to Prof. Dr. Sebahattin Ozcan, University of Ankara for providing recombinant plasmid used in the study.
dc.identifier.doi10.3906/tar-1910-13
dc.identifier.endpage407
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85088991148
dc.identifier.scopusqualityQ2
dc.identifier.startpage399
dc.identifier.trdizinid353294
dc.identifier.urihttps://doi.org/10.3906/tar-1910-13
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/353294
dc.identifier.urihttps://hdl.handle.net/11480/14198
dc.identifier.volume44
dc.identifier.wosWOS:000555548000008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectDiploid potatoes
dc.subjectgenetic improvement
dc.subjectAgrobacterium-mediated transformation
dc.subjectSolanum chacoense M6
dc.titleManipulating genome of diploid potato inbred line Solanum chacoense M6 using selectable marker gene
dc.typeArticle

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