Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses

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dc.authoridYALCIN, MELIS/0000-0002-6336-4018
dc.authoridOZTURK, ZAHIDE NESLIHAN/0000-0002-2592-9997
dc.contributor.authorYalcin, Melis
dc.contributor.authorOzturk Gokce, Zahide Neslihan
dc.date.accessioned2024-11-07T13:31:33Z
dc.date.available2024-11-07T13:31:33Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPotato (Solanum tuberosum L.) growth, development and production are sensitive to abiotic stress conditions, yet studies about investigation of stress response of potato plants in regulation of gene expression level are limited. The aim of this study was to identify the role of newly identified Novel_105 miRNA in contrasting potato cultivars (tolerant Unica and sensitive Russet Burbank) in response to heat, drought and combined heat and drought stresses using transgenic approach. Transgenic plants of Unica and Russet Burbank were generated with overexpression of pre-miRNA of Novel_105 and both wild type and transgenic T0 lines were subjected to stress. Physiological parameters (gaseous exchange, leaf temperature, chlorophyll content and relative water content) were observed along with biochemical parameters including proline, malondialdehyde and hydrogen peroxide. The overexpression of Novel_105 improved physiobiochemical functions of the both cultivars under single or combined stress conditions. The increase in Novel_105 miRNA expression was observed along with a decreased expression in its predicted target E3 ubiquitin-protein ligase XBAT35, that is responsible for cell death in potato. This is a preliminary study for the development of abiotic stress resilient potato cultivars proving the overexpression of Novel_105 miRNA increased abiotic stress tolerance presumably by repressing the expression of XBAT35 responsible from promoting cell death.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [115o405]
dc.description.sponsorshipThis project was completed with the funding of the Scientific and Technological Research Council of Turkey (TUBITAK) (Project Number, 115o405). We would like to acknowledge Arslan Asim, Esra Kaplan, Hussain Azimi, Ilknur Tindas and Beyazit Sanli who worked as scholars in the project. Our great appreciation goes to Usman Khalid Chaudhry and Muhammad Daniyal Junaid who helped in data analyses and writing of the manuscript. We also acknowledge project members Sevgi Caliskan, Mehmet Emin Caliskan, Ufuk Demirel, Allah Bakhsh and Emre Aksoy for their contribution to the studies.
dc.identifier.doi10.3906/bot-2103-39
dc.identifier.endpage+
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85117226288
dc.identifier.scopusqualityQ2
dc.identifier.startpage397
dc.identifier.trdizinid514220
dc.identifier.urihttps://doi.org/10.3906/bot-2103-39
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/514220
dc.identifier.urihttps://hdl.handle.net/11480/14916
dc.identifier.volume45
dc.identifier.wosWOS:000702440500002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectDrought
dc.subjectheat
dc.subjectcombined heat and drought stresses
dc.subjectNovel_105
dc.subjecttransgenic overexpression
dc.subjectpotato
dc.titleInvestigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses
dc.typeArticle

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