Characterization of SE-P3, P16, P37, and P47 bacteriophages infecting Salmonella Enteritidis

Küçük Resim Yok

Tarih

2019

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Wiley

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

The aim of this study was to identify and characterize the SE-P3, P16, P37, and P47 phages infecting Salmonella Enteritidis. Transmission electron microscopy analysis showed that the SE phages belonged to the Myoviridae or Siphoviridae family and had plaque sizes between 0.622 +/- 0.027 and 1.630 +/- 0.036mm in diameter. sefC, pefA, spvC, sopE, and gipA virulent gene regions were absent in their genome and their calculated genome sizes were between 35.9 and 37.8 kbp. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the protein profiles of each phage were different. The SE phages had a short latent period (10-20 min), large burst size (76-356 PFU/cell), and a short burst time (25-35 min). The multiplicity of infection values and mutant frequency of the phages were 0.01-0.0001 and 10(-7), respectively. They were very infective against their host bacteria when applied at 20 degrees C, 30 degrees C, or 37 degrees C and adsorbed to their host cells by 96.20-97.65% in the first 5 min of incubation, and also Ca2+ ions did not have a significant effect on their adsorption. The SE phages were resistant to wide pH ranges and high temperatures. These results indicate that the SE phages are good candidates as therapeutic and biocontrol agents against foodborne pathogenic S. Enteritidis.

Açıklama

Anahtar Kelimeler

bacteriophage, characterization, morphology, Salmonella Enteritidis, virulent gene

Kaynak

Journal of Basic Microbiology

WoS Q Değeri

Q3

Scopus Q Değeri

Q2

Cilt

59

Sayı

10

Künye