Characterization of SE-P3, P16, P37, and P47 bacteriophages infecting Salmonella Enteritidis

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dc.contributor.authorYildirim, Zeliha
dc.contributor.authorSakin, Tuba
dc.contributor.authorAkcelik, Mustafa
dc.contributor.authorAkcelik, Nesife
dc.date.accessioned2024-11-07T13:32:23Z
dc.date.available2024-11-07T13:32:23Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe aim of this study was to identify and characterize the SE-P3, P16, P37, and P47 phages infecting Salmonella Enteritidis. Transmission electron microscopy analysis showed that the SE phages belonged to the Myoviridae or Siphoviridae family and had plaque sizes between 0.622 +/- 0.027 and 1.630 +/- 0.036mm in diameter. sefC, pefA, spvC, sopE, and gipA virulent gene regions were absent in their genome and their calculated genome sizes were between 35.9 and 37.8 kbp. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the protein profiles of each phage were different. The SE phages had a short latent period (10-20 min), large burst size (76-356 PFU/cell), and a short burst time (25-35 min). The multiplicity of infection values and mutant frequency of the phages were 0.01-0.0001 and 10(-7), respectively. They were very infective against their host bacteria when applied at 20 degrees C, 30 degrees C, or 37 degrees C and adsorbed to their host cells by 96.20-97.65% in the first 5 min of incubation, and also Ca2+ ions did not have a significant effect on their adsorption. The SE phages were resistant to wide pH ranges and high temperatures. These results indicate that the SE phages are good candidates as therapeutic and biocontrol agents against foodborne pathogenic S. Enteritidis.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey (TUBITAK)
dc.identifier.doi10.1002/jobm.201900102
dc.identifier.endpage1062
dc.identifier.issn0233-111X
dc.identifier.issn1521-4028
dc.identifier.issue10
dc.identifier.pmid31347183
dc.identifier.scopus2-s2.0-85072992345
dc.identifier.scopusqualityQ2
dc.identifier.startpage1049
dc.identifier.urihttps://doi.org/10.1002/jobm.201900102
dc.identifier.urihttps://hdl.handle.net/11480/15362
dc.identifier.volume59
dc.identifier.wosWOS:000478200800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Basic Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectbacteriophage
dc.subjectcharacterization
dc.subjectmorphology
dc.subjectSalmonella Enteritidis
dc.subjectvirulent gene
dc.titleCharacterization of SE-P3, P16, P37, and P47 bacteriophages infecting Salmonella Enteritidis
dc.typeArticle

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