The Effect of Sex-Specific Genetic Factors on the Host Immune Response to COVID-19: A Pilot Study

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dc.authoridYILDIZ, ORHAN/0000-0001-6019-0430
dc.authoridKayabas, Uner/0000-0002-5323-0796
dc.authorid, Zeynep Ture/0000-0001-6895-0318
dc.contributor.authorBali, Dilara Fatma Akin
dc.contributor.authorGulen, Tugba Arslan
dc.contributor.authorOzmen, Esma
dc.contributor.authorYuce, Zeynep Ture
dc.contributor.authorYildiz, Orhan
dc.contributor.authorTurunc, Tuba
dc.contributor.authorKayabas, Uner
dc.date.accessioned2024-11-07T13:34:38Z
dc.date.available2024-11-07T13:34:38Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjective: The aim of this study was to investigate the impact of sex-specific genetic factors in the pathogenesis and prog-nosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2)-induced macrophage activation syndrome (MAS), independent of age and comorbidity presence.Materials and Methods: Patients aged 18-50 years who had been diagnosed with coronavirus 2019 (COVID-19), the disease caused by the SARS-CoV2 virus, were enrolled in a prospective, case-control, multi-center study. Genetic alterations and messenger RNA (m-RNA) expression levels of the TLR7, TLR8, ACE2, CD40L, CXCR3, and TASL genes were determined using DNA sequencing analysis, and gene expression was determined using quantitative reverse transcriptase polymerase chain reaction testing. PolyPhen-2 (Polymorphism Phenotyping v2; Adzhubei et al., 2010) and SNAP2 (Rostlab, Munich, Germany) genetic analysis tools were used to define the pathogenic effects of detected mutations by sequencing the selected genes in hotspot regions.Results: The study group consisted of 80 patients diagnosed with COVID-19 and was divided into groups based on sex and MAS status. Twenty-nine mutations were detected in 6 genes. Among the alterations, 15 were identified in this study for the first time and 9 were pathogenic. Pathogenic missense mutations in the TLR7, TLR8, ACE2, and TASL genes were detected in the MAS (+) group. In males, decreased TLR7, TLR8, and CXCR3 expression was statistically significant in the MAS (+) group (p<0.050). CXCR3 expression was lower in the female and male MAS (+) groups compared with the MAS (-) groups (p<0.050).Conclusion: In the absence of major risk factors for COVID-19, the TLR7/8, ACE2, and CXCR3 variants and decreased m-RNA expression levels associated with genetic susceptibility may be independent prognostic risk factors for COVID-19.
dc.description.sponsorshipScientific Research Projects of Nidemer Halisdemir University (BAP) [SAT 2021/2-NAP]
dc.description.sponsorshipThis work was supported by grants from the Scientific Research Projects of Nidemer Halisdemir University (BAP; Project no SAT 2021/2-NAP) .
dc.identifier.doi10.14744/etd.2022.32955
dc.identifier.endpage602
dc.identifier.issn2149-2247
dc.identifier.issn2149-2549
dc.identifier.issue6
dc.identifier.startpage594
dc.identifier.trdizinid1173249
dc.identifier.urihttps://doi.org/10.14744/etd.2022.32955
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1173249
dc.identifier.urihttps://hdl.handle.net/11480/16079
dc.identifier.volume44
dc.identifier.wosWOS:000885122500009
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherErciyes Univ Sch Medicine
dc.relation.ispartofErciyes Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectACE2
dc.subjectCOVID-19
dc.subjecthost genetics
dc.subjectmutation
dc.subjectsex difference
dc.subjectTLR7
dc.subjectX chromosome
dc.titleThe Effect of Sex-Specific Genetic Factors on the Host Immune Response to COVID-19: A Pilot Study
dc.typeArticle

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