Niğde mezbahasında kesilen koyunlarda anti-toxoplasma gondii antikorlarının ELISA testi ile araştırılması
Küçük Resim Yok
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Niğde Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışma, Ocak-Nisan 2011 tarihleri arasında Niğde mezbahasında kesilen koyunlarda anti-Toxoplasma gondii antikorlarının seroprevalansının ELISA testi ile belirlenmesi amacı ile yapılmıştır. Bu amaçla; Ocak ayında 34, Şubat ayında 60, Mart ayında 40 ve Nisan ayında 41 koyun olmak üzere 1 yaş ve üzerindeki toplam 175 koyundan kan alınmıştır. Serumları çıkarılan kan örnekleri ELISA testi ile incelenmiş ve toplam 175 koyunun 11 (%6.28)'inde anti-T. gondii antikorları saptanmıştır. Koyunların serolojik incelemesinde en yüksek seropozitiflik oranı %12.5 (5/40) ile Mart ayında belirlenirken, Ocak ayında %5.9 (2/34), Şubat ayında %6.7 (4/60) oranında seropozitiflik belirlenmiştir. Buna karşılık Nisan ayında muayene edilen koyunların hiçbirinde T. gondii seropozitifliği tespit edilememiştir. Aylar açısından koyunlardaki seropozitiflik oranları karşılaştırıldığında istatistiksel olarak bir farklılık bulunamamıştır (p>0.05). Sonuç olarak, Niğde yöresinde mezbahada kesilen koyunlarda T. gondii'nin varlığı ortaya konulmuştur.Anahtar sözcükler: Toxoplasma gondii, Koyun, Niğde, Mezbaha, Seroprevalans, ELISA
The objective of this study is to determine seroprevalence of anti-Toxoplasma gondii antibodies with ELISA test in the sheep slaughtered in Nigde slaughterhouse. The study was carried out between January and April 2011, where blood samples were taken from 175 sheep which were a year old or more. In January 34, in February 60, in March 40, and in April 41 blood samples were collected. The serum samples obtained from the blood samples were examined with ELISA test, and anti-T. gondii antibodies were detected in 11 out of the 175 samples (6.28%). Serological examination of the sheep shows that the highest seropositivity rate of 12.5% (5/40) was detected in March, while the seropositivity rate in January was 5.9% (2/34), and in February it was 6.7% (4/60). However, none of the sheep inspected in April tested seropositive for T. gondii. Comparison of the seropositivity rates of the sheep with regards to months did not reveal statistical significance (p>0.05). In conclusion, the presence and the rate of T.gondii in the sheep slaughtered in Niğde region have been determined.Keywords: Toxoplasma gondii, Sheep, Nigde, Slaughterhouse, Seroprevalence, ELISA
The objective of this study is to determine seroprevalence of anti-Toxoplasma gondii antibodies with ELISA test in the sheep slaughtered in Nigde slaughterhouse. The study was carried out between January and April 2011, where blood samples were taken from 175 sheep which were a year old or more. In January 34, in February 60, in March 40, and in April 41 blood samples were collected. The serum samples obtained from the blood samples were examined with ELISA test, and anti-T. gondii antibodies were detected in 11 out of the 175 samples (6.28%). Serological examination of the sheep shows that the highest seropositivity rate of 12.5% (5/40) was detected in March, while the seropositivity rate in January was 5.9% (2/34), and in February it was 6.7% (4/60). However, none of the sheep inspected in April tested seropositive for T. gondii. Comparison of the seropositivity rates of the sheep with regards to months did not reveal statistical significance (p>0.05). In conclusion, the presence and the rate of T.gondii in the sheep slaughtered in Niğde region have been determined.Keywords: Toxoplasma gondii, Sheep, Nigde, Slaughterhouse, Seroprevalence, ELISA
Açıklama
Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalı
Anahtar Kelimeler
Biyoloji, Biology, Parazitoloji
