Investigation of CTLA-4 gene +49 A/G polymorphism in patients with bladder cancer
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Cytotoxic T-lymphocyte antigen 4 (CTLA-4) is a member of the immunoglobulin superfamily. This stimulator molecule is expressed by active T cells. Recent studies have determined that dysfunction of CTLA-4 antigen leads to various diseases (Behçet's disease, diabetes, Graves' disease and systemic lupus erythematosus). It has also been suggested that there may be a relationship between the CTLA-4 gene and various cancers (breast cancer, osteosarcoma, and cervical cancer). In this study, It was investigated whether the CTLA-4 gene +49 A/G (rs231775) polymorphism could be assessed as a risk factor for bladder cancer patients. Material and Methods: This study included 69 patients with bladder cancer (62.07 ± 10.48 years) with similar age distribution and 149 healthy control (61.54 ± 14.43 years). Genotypes were determined by the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Data was analysed using the Chi-square test and Hardy-Weinberg test. The Hardy-Weinberg equilibrium test was used to test the distributions of genotype frequency. Results: AA genotype was found in 41 (60%, in patients), 81 (55%, in control), AG genotype 23 (33%), 54 (36%) and GG genotype 5 (7%), 14 (9%) respectively in patients with bladder cancer and in healthy control groups. The frequency of GG genotype in patients was less than controls, but there was no statistically significant difference in the frequencies of GG genotype between patients and controls (P=0.52). Conclusions: These results indicated that genotype and allele frequencies of CTLA-4 gene +49 A/G polymorphism were not statistically significant different between bladder cancer patients and control groups. © 2017 by Türkiye Klinikleri.
Açıklama
Anahtar Kelimeler
Ctla-4 antigen, Genetic, Polymerase chain reaction, Polymorphism, Urinary bladder neoplasms
Kaynak
Turkiye Klinikleri Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
37
Sayı
1
