Investigation the potential use of silver nanoparticles synthesized by propolis extract as N-acyl-homoserine lactone-mediated quorum sensing systems inhibitor

Basit Öğe Kaydı
dc.authoridSAGLAM, NECDET/0000-0002-5463-8355
dc.authoridEmul, Ezgi/0000-0003-3834-2260
dc.authoridIlk, Sedef/0000-0002-6754-792X
dc.contributor.authorIlk, Sedef
dc.contributor.authorTan, Gamze
dc.contributor.authorEmul, Ezgi
dc.contributor.authorSaglam, Necdet
dc.date.accessioned2024-11-07T13:24:05Z
dc.date.available2024-11-07T13:24:05Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.descriptionTaiwan-Turkey Science Summit on Translation of Cells, Nanomaterials and Signaling Molecules into Regenerative Medicine -- APR 01-03, 2018 -- Ankara, TURKEY
dc.description.abstractBackground/aim: Quorum sensing (QS) is a chemical communication process that bacteria use to regulate virulence. Inhibition of QS (antiQS) overcomes the pathogenicity of bacteria. Silver nanoparticles (AgNPs) have been used as antimicrobials against pathogens, but have not been used against QS-mediated bacterial infection. Also, studies have been carried out on the inhibitory effects of propolis based structures on pathogen growth, but no studies have been found on their potential use as QS inhibitor. The present study aims to investigate the synthesis and characterization of silver nanoparticles (AgNPs) reduced with propolis extract (P-AgNPs) and evaluation of their antimicrobial and, for the first time, antiQS activity. Materials and methods: P-AgNPs were synthesized using with different volumes (1, 2.5 and 5 mL) of propolis extract (PE) by biological method via reduction of silver nitrate. Synthesized P-AgNPs were characterized in terms of hydrodynamic, chemical, morphological, physical, and antioxidant properties. Disc diffusion and flask incubation assays were used to evaluate the antimicrobial effect against Gram-negative bacteria (Escherichia coli, Proteus mirabilis, Proteus vulgaris, Salmonella typhimurium, Enterobacter aerogenes, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus mutans, Bacillus thuringiensis) and QS-regulated biofilm activity against biosensor strain Chromobacterium violaceum CV026. Results: AgNPs were successfully synthesized by biological method via PE. The violacein pigment production based on the QS system was greatly inhibited by the P-AgNPs (inhibition zones: 16.22-21.48 mm and violacein inhibition: 63.16 +/- 2.4-75.24 +/- 3.5 %) without interfering with the growth of bacteria, which is the first report on the antiQS effect of P-AgNPs. Conclusion: Our results suggest that P-AgNPs may be potentially used to inhibit bacterial physiological processes due to the signal molecules regulates important collective behavior of bacteria. The development of such nontoxic biomaterials may have great potential to evaluate for the new medicinal substance that inhibits the pathogenic biofilms.
dc.identifier.doi10.3906/sag-2004-148
dc.identifier.endpage1156
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue4
dc.identifier.pmid32304193
dc.identifier.scopus2-s2.0-85090750853
dc.identifier.scopusqualityQ3
dc.identifier.startpage1147
dc.identifier.trdizinid354783
dc.identifier.urihttps://doi.org/10.3906/sag-2004-148
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/354783
dc.identifier.urihttps://hdl.handle.net/11480/13904
dc.identifier.volume50
dc.identifier.wosWOS:000549845300010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectsilver nanoparticles
dc.subjectquorum sensing
dc.subjectvirulence factor
dc.subjectantimicrobial agent
dc.subjectpropolis
dc.titleInvestigation the potential use of silver nanoparticles synthesized by propolis extract as N-acyl-homoserine lactone-mediated quorum sensing systems inhibitor
dc.typeConference Object

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu