Are VEGF and SCUBE1 gene expressions increased in diabetic retinopathy?

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dc.authoridayan, durmus/0000-0003-2615-8474
dc.authoridOnder, Cagatay Emir/0000-0002-0293-2309
dc.contributor.authorAyan, Durmus
dc.contributor.authorZor, Kuersad Ramazan
dc.contributor.authorOzmen, Esma
dc.contributor.authorBicer, Gamze Yildirim
dc.contributor.authorOnder, Cagatay Emir
dc.contributor.authorSari, Ismail
dc.date.accessioned2024-11-07T13:31:30Z
dc.date.available2024-11-07T13:31:30Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjectives: In the current study, we synergistically evaluated vascular endothelial growth factor (VEGF) gene expression levels and signal peptide-CUB-EGF domain-containing protein 1 (SCUBE1) gene expression levels in diabetic patients without retinopathy, non-proliferative diabetic retinopathy (NPDR), and proliferative diabetic retinopathy (PDR).Methods: 94 blood samples from 26 healthy controls, 29 non-DR, 22 NPDR, and 17 PDR patients were collected in sterile EDTA tubes. Total RNA was obtained from these samples without waiting and then converted to cDNA. The expression levels of the VEGF and SCUBE1 genes were determined by quantitative real-time polymerase chain re-action (qPCR).Results: SCUBE1 gene expression levels were 2.15 (p=0.015), 1.75 (p=0.799), 2.37 (p=0.037) times higher, and VEGF gene expression levels were 1.71 (p=0.023), 1.75 (p=0.012), 1.85 (p=0.031) times higher in the non-DR, NPDR, and PDR groups compared to the control group, respectively. VEGF gene expression levels were significantly higher in participants with HbA1c levels >= 5.7% compared to those with < 5.7. SCUBE1 and VEGF gene expression levels were significantly higher in participants with fasting plasma glucose (FPG) levels >= 126 mg/dL than those with < 126 mg/dL.Conclusions: As a result, SCUBE1 gene expression levels are higher than VEGF gene expression levels, especially in the PDR group. Therefore, SCUBE1 may contribute to the pathology of DR just like VEGF by generating angiogenesis. However, we believe there isa need for experimental animal model studies with DR examining SCUBE1 gene expression levels in tissue samples.
dc.identifier.doi10.1515/tjb-2023-0008
dc.identifier.endpage57
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85147905599
dc.identifier.scopusqualityQ4
dc.identifier.startpage51
dc.identifier.trdizinid1252498
dc.identifier.urihttps://doi.org/10.1515/tjb-2023-0008
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1252498
dc.identifier.urihttps://hdl.handle.net/11480/14866
dc.identifier.volume48
dc.identifier.wosWOS:000925138800001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectdiabetes
dc.subjectdiabetic retinopathy
dc.subjectSCUBE1
dc.subjectVEGF
dc.titleAre VEGF and SCUBE1 gene expressions increased in diabetic retinopathy?
dc.typeArticle

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