Wolbachia spp. and Spiroplasma spp. in Musca spp.: Detection Using Molecular Approaches

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dc.contributor.authorKaratepe, Mustafa
dc.contributor.authorAksoy, Serap
dc.contributor.authorKaratepe, Bilge
dc.date.accessioned2024-11-07T10:39:52Z
dc.date.available2024-11-07T10:39:52Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjective: This study aimed to detect the presence of Wolbachia and Spiroplasma endosymbionts in Musca flies through molecular approaches. Methods: In total, 40 Musca spp. (20 female and 20 male) were used. Before DNA extraction, the flies were dissected and their heads, wings and legs were detached from their bodies under a stereomicroscope. Genomic DNA was analysed by standard polymerase chain reaction (PCR) using primers against Musca beta-tubulin. Afterward, the samples were examined for the presence of Wolbachia spp. using primers against Wolbachia wsp and GroEL. Furthermore, the DNA samples were analysed by PCR to detect the presence of Spiroplasma using primers against the 16S rRNA. Results: No Wolbachia positivity was detected in Musca flies, as shown by the negative PCR results for wsp and GroEL. Spiroplasma positivity was detected in 5% (1/20) of the female Musca flies but not in the male flies (0/20). Conclusion: Wolbachia spp. were not detected in Musca flies. Of the total Musca flies, only one was positive for Spiroplasma spp. To our knowledge, this is the first study to detect the presence of Spiroplasma in Musca flies. © 2021 Turkish Society for Parasitology.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (BIDEB-2219)
dc.identifier.doi10.4274/tpd.galenos.2021.35229
dc.identifier.endpage215
dc.identifier.issn1300-6320
dc.identifier.issue3
dc.identifier.pmid34346878
dc.identifier.scopus2-s2.0-85114055339
dc.identifier.scopusqualityQ4
dc.identifier.startpage211
dc.identifier.trdizinid481630
dc.identifier.urihttps://doi.org/10.4274/tpd.galenos.2021.35229
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/481630
dc.identifier.urihttps://hdl.handle.net/11480/11242
dc.identifier.volume45
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherGalenos Publishing House
dc.relation.ispartofTurkiye Parazitoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectMusca
dc.subjectPCR
dc.subjectSpiroplasma
dc.subjectWolbachia
dc.titleWolbachia spp. and Spiroplasma spp. in Musca spp.: Detection Using Molecular Approaches
dc.title.alternativeMusca Türlerinde Wolbachia spp. ve Spiroplasma spp.’nin Moleküler Yöntemlerle İncelenmesi
dc.typeArticle

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